“嗅三针”疗法对血管性痴呆大鼠学习记忆功能及Nrf2表达的影响  

Effects of “Three Olfactory Acupoints” Therapy on Learning and Memory Function and Nrf2 Expression in Vascular Dementia Rats

作　　者：李杰[1,2] 王渊[2] 鲁刚[2] 牛文民[2] 王强[2] Li Jie;Wang Yuan;Lu Gang;Niu Wenmin;Wang Qiang(Chengdu University of Chinese Medicine,Chengdu China,610075;Shaanxi University of Chinese Medicine,Xianyang China,712046)

机构地区：[1]成都中医药大学,四川成都610075 [2]陕西中医药大学,陕西西咸新区712046

出　　处：《现代中医药》2020年第2期29-32,共4页Modern Chinese Medicine

基　　金：国家自然科学基金面上项目(81674086);陕西中医药大学课题(2017SZKY-041)。

摘　　要：目的研究"嗅三针"疗法对VD模型大鼠的干预作用及其与海马Nrf2蛋白含量的关系。方法选取45只成年SD雄性大鼠,体重为200~220g。随机分为3组,即正常组、模型组(VD模型组)、嗅三针组,每组15只。制作模型,运用嗅三针进行干预治疗,然后,运用水迷宫实验测试大鼠学习记忆能力,并采用western blot法、ELISA等法测定海马Nrf2蛋白含量。结果水迷宫试验结果显示关于平均游泳路程和平均逃避潜伏期数据统计,模型组大鼠逃脱潜伏期增加(定位航行实验)、目标平台象限占用平均时间减少,与空白组大鼠比较具有显著性差异(P﹤0.01);经"嗅三针"干预后,大鼠逃脱潜伏期减少、目标平台象限占用平均时间增加,与模型组比较具有显著性差异(P﹤0.05);与空白组大鼠比较,模型组大鼠Nrf2表达增加,具有显著性差异(P﹤0.01);"嗅三针"组与模型组比较,Nrf2表达增加,与模型组比较具有显著性差异(P﹤0.05)。结论 "嗅三针"疗法可明显改善VD大鼠的学习记忆能力,该疗效机理与大脑海马Nrf2的激活密切相关。Objective To study the intervention effect of "three olfactory acupoints" therapy on VD model rats and its relationship with hippocampal Nrf2 protein content. Methods Forty-five adult male SD rats were selected, weighing 200-220 g. They were randomly divided into three groups, namely the normal group, the model group(VD model group), and "three olfactory acupoints" group, with 15 in each.The model was made, and three olfactory acupoints were used for intervention treatment. Then, the water maze experiment was used to test the learning and memory ability of the rats, and the western blot method and ELISA were used to determine the hippocampal Nrf2 protein content. Results The results of the water maze test showed that, as for the average swimming distance and average escape latency, the escape latency of the model group was increased(positioning navigation experiment), the average time of the target platform quadrant occupied decreased, which was significantly different from the blank group(P < 0.01);after " three olfactory acupoints" intervention, the escape latency of the rats was reduced, and the average time spent on the target platform quadrant was increased, which was significantly different from that of the model group(P <0.05);compared with the rats of the blank group The expression of Nrf2 in the model group increased significantly, with a significant difference(P <0.01);compared with the model group, the expression of Nrf2 increased in the "three olfactory acupoints" group, which had a significant difference compared with the model group(P <0.05). Conclusion " Three olfactory acupoints" therapy can significantly improve the learning and memory ability of VD rats, and the mechanism is closely related to the activation of Nrf2 in the hippocampus of the brain.

关 键 词：嗅三针 血管性痴呆 NRF2 

分 类 号：R245[医药卫生—针灸推拿学]