爵床提取物抗炎镇痛作用的实验研究  

EXPERIMENTAL STUDIES ON THE ANTI-INFLAMMATORY AND ANALGESIC EFFECT OF ROSTELLULARIA PROCUMBENS EXTRACT

作　　者：梁生林 钟卫华 李庆耀 褚洪标 周细根 LIANG Sheng-lin;ZHONG Wei-hua;LI Qing-yao;CHU Hong-biao;ZHOU Xi-gen(Health Science Center,Jinggangshan University,Ji’an,Jiangxi 343009,China;Affiliated Hospital,Jinggangshan University,Ji’an,Jiangxi 343000,China)

机构地区：[1]井冈山大学医学部,江西吉安343009 [2]井冈山大学附属医院,江西吉安343000

出　　处：《井冈山大学学报（自然科学版）》2020年第1期86-92,共7页Journal of Jinggangshan University (Natural Science)

基　　金：国家科技支撑计划项目子题项目(2012BAC11B02-6);吉安市科技支撑计划项目(吉市科计字[2014]4号);全国中药资源普查项目(财社[2018]43号)。

摘　　要：目的探讨爵床提取物的抗炎镇痛作用。方法采用致炎剂二甲苯引起小鼠耳肿胀、新鲜蛋清引起大鼠足跖肿胀2种急性炎症模型以及棉球引起大鼠肉芽肿慢性炎症模型,研究爵床提取物灌胃给药对急慢性炎症的作用。采用热板法、醋酸扭体法和甲醛法致痛,研究爵床提取物灌胃给药的镇痛作用。结果与纯化水对照组相比,爵床醇提取物和水提取物高、低剂量组对致炎剂二甲苯所引起的小鼠耳廓肿胀抑制显著(P<0.01,P<0.05),对蛋清所引起的大鼠足跖肿胀抑制显著(P<0.01),对大鼠棉球肉芽肿抑制显著(P<0.01)。爵床醇提取物和水提取物高、低剂量组给药后对热板所引起的小鼠痛阈值明显提高,与纯化水对照组和自身给药前比较差异明显(P<0.01,P<0.05);爵床醇提取物和水提取物高、低剂量组对致痛剂醋酸所引起的小鼠扭体反应次数明显减少,也明显抑制甲醛所引起的早期(0~5 min)和晚期(15~30 min)小鼠足痛反应,与纯化水对照组比较差异显著(P<0.01)。爵床醇提取物的抗炎镇痛作用比水提取物强(P<0.01,P<0.05)。结论爵床提取物灌胃给药具有一定抗炎、镇痛作用,且醇提取物强于水提取物。Objective:To investigate the anti-inflammatory and analgesic effects of Rostellularia procumbens extract.Methods:The anti-inflammatory animal models were used in researching the effect of R.procumbens extract on acute and chronic inflammation.The acute models were xylene-induced ear edema and egg white-induced paw edema,while the chronic one was granuloma induced by cotton pellet implantation.Meanwhile,the analgesic effect of the extract was observed by hot plate acetic,acid writhing test and the formalin induced-pain experiment.Results:Compared with the purified water control group,high and low doses of R.procumbens alcohol and water extract could significantly inhibit ear edema in mice(P<0.01,P<0.05)and the paw edema in rat(P<0.01).Also,the alcohol and water extract decreased the granuloma of rats(P<0.01).Moreover,the pain threshold with hot plate method was significantly increased(P<0.01,P<0.05)compared with the purified water control group and the data before the drugs administration of high and low doses of R.procumbens alcohol and water extract.And the writhing number was reduced(P<0.01)when contrasted to the purified water control group after extract administration.The alcohol and water extract also significantly inhibited foot pain response at early phase(0-5 min)and late phase(15-30 min)caused by formalin in mice(P<0.01).However,there were substantial variations in the alcohol extract groups and the same dose of water extract in high and low concentrations(P<0.01,P<0.05).Conclusion:R.procumbens extract possesses the anti-inflammatory and analgesic effect,alcohol extract has better effect than water extract.

关 键 词：爵床 醇提取物和水提取物 抗炎 镇痛 

分 类 号：R285.5[医药卫生—中药学]