巴戟天内生真菌Trichoderma spirale A725中两个新的聚酮类化合物(英文)  

Two New Polyketide Compounds from the Endophytic Fungus Trichoderma spirale A725 of Morinda officinalis

作　　者：陈书帅 刘洪新[2] 刘昭明 李赛妮 陈玉婵[2] 李浩华[2] 李冬利 章卫民[2] Chen Shushuai;Liu Hongxin;Liu Zhaoming;Li Saini;Chen Yuchan;Li Haohua;Li Dongli;Zhang Weimin(School of Biotechnology and Health Sciences,Wiyi University,Jiangmen,Guangdong 529020;State Key Laboratory of Applied Microbiology Souuhern China,Guangdong Provincial Key Laboratory of Microbial Cuture Collection and Application,Guangdong Open Laboratory of Applied Microbiology,Guangdong Instiute of Microbiology,Guangdong Academy of Sciences,Guangzhou 510070)

机构地区：[1]五邑大学生物科技与大健康学院,广东江门529020 [2]广东省微生物研究所,广东省科学院,华南应用微生物国家重点实验室,广东省菌种保藏与应用重点实验室,广东省微生物应用新技术公共实验室,广州510075

出　　处：《有机化学》2020年第1期209-214,共6页Chinese Journal of Organic Chemistry

基　　金：国家自然科学基金(No.31600271);广东省科技计划(No.2107A020211023);广州市珠江科技新星(No.201806010080)资助项目。

摘　　要：综合运用硅胶柱色谱、反相硅胶柱色谱、Sephadex LH-20凝胶柱色谱以及制备型高效液相色谱技术对药用植物巴戟天内生真菌Trichoderma spirale A725的次生代谢产物进行分离纯化,得到6个聚酮类化合物,采用多种现代波谱技术确定其结构,分别为:6-羟基-4-异丙基-1,8-二甲基螺环[4.5]癸-1,8-二烯-7-酮(1),2-羟基-2,5-二甲基-7-氧代-5,7-二氢-2H-呋喃[3,4-b]吡喃-4-羧酸(2), 3-乙基-4-羟基-6-甲基-二氢-吡喃-2-酮(3),苯乙内酯A (4), 3-羟基-5-(4-羟基苄基)二氢呋喃-2(3H)-酮(5), 4-乙酰-3-羟基-6-甲基吡喃-2-酮(6).其中化合物1和2为新化合物,化合物3为新天然产物.此外,利用四株肿瘤细胞株(Hep G-2、MCF-7、SF-268及A549)对化合物1~6细胞毒活性进行评估,结果表明化合物1~6对上述肿瘤细胞均无明显的细胞毒活性.Six polyketides were isolated from the fermentation broth of an endophytic fungus Trichoderma spirale A725 of the medicinal plant Morinda officinalis by silica gel, reversed phase silica column chromatography, sephadex LH-20 and high performance preparative liquid chromatography. Their structures were elucidated to be 6-hydroxy-4-isopropyl-1,8-dimethylspiro[4.5]deca-1,8-dien-7-one(1), 2-hydroxy-2,5-dimethyl-7-oxo-5,7-dihydro-2 H-furo[3,4-b]pyran-4-carboxylicacid(2), 3-ethyl-4-hydroxy-6-methyl-2 H-pyran-2-one(3), harzialactone A(4), 3-hydroxy-5-(4-hydroxybenzyl)dihydrofuran-2(3 H)-one(5), and 4-acetyl-3-hydroxy-6-methyl-pyran-2-one(6) on the basis of extensive spectral analysis. Among them, compounds 1 and 2 were new compounds, and compound 3 was a new natural product. Moreover, the cytotoxicities of compounds 1~6 were evaluated against four cancer cell lines(Hep G-2, MCF-7, SF-268 and A549). However, none of them exhibited cytotoxic activity against the tested tumor cells.

关 键 词：巴戟天 内生真菌 螺旋木霉 聚酮 

分 类 号：O657.63[理学—分析化学] R284.2[理学—化学] R285.5[医药卫生—中药学]