水曲柳FmJAZ1基因在非生物胁迫中的响应模式和激素诱导表达分析  

Analysis of Expression of FmJAZ1 Gene in Fraxinus mandshurica:Response Model to Abiotic Stresses and Hormone Induction

作　　者：屈申 刘宗林 杨少彤 于磊[1,2] 何利明[1] 韩丹宇 詹亚光[1,2] QU Shen;LIU Zong-lin;YANG Shao-tong;YU Lei;HE Li-ming;HAN Dan-yu;ZHAN Ya-guang(College of Life Science,Northeast Forestry University,Harbin 150040;Northeast Forestry Universit/State Key Laboratory of Tree Genetics and Breeding,Harbin 150040)

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040 [2]东北林业大学/国家林木遗传育种国家重点实验室,哈尔滨150040

出　　处：《植物遗传资源学报》2020年第2期500-506,共7页Journal of Plant Genetic Resources

基　　金：国家重点研发计划课题(2017YFD0600605-01);大学生创新课题(201810225127)。

摘　　要：克隆FmJAZ1基因,明确其在低温和NaCl胁迫中的响应模式和激素诱导下的转录表达特性。通过基因克隆的方法得到水曲柳中的FmJAZ1基因,利用生物信息学软件对所得到的序列进行分析并构建系统进化树,对水曲柳FmJAZ1基因进行了时空表达特异性的分析,对根、茎、叶、芽、雄花、雌花、种子等7个部位以及在5-9月5个月份分别取样,对水曲柳进行低温(4℃)和盐胁迫(200 mmol/L NaCl)2种非生物胁迫处理以及脱落酸(ABA)、赤霉素(GA3)、生长素(IAA)、茉莉酸(JA)、水杨酸(SA)等激素信号诱导处理,然后对试验材料进行荧光定量分析。克隆出全长为684 bp的核苷酸序列。生物信息学软件分析得到JZA1基因具有完整的开放阅读框,编码227个氨基酸,JAZ1蛋白不含有信号肽,不属于跨膜蛋白,为不稳定亲水性蛋白。时空表达结果显示,FmJAZ1基因在茎中表达量最高,且在8月份表达量最高;非生物胁迫结果表明低温处理后FmJAZ1在6h、24h表达量较高;而NaCl处理后,在24 h表达量较高,且该基因响应低温胁迫较NaCl胁迫迅速;激素信号诱导结果显示,处理后不同时间,基因表达量变化较为明显,其中GA3处理后3h最为明显,为对照组的77.3倍,分析了FmJAZ1基因在低温、NaCl胁迫和激素诱导下的表达模式。FmJAZ1基因充分响应了逆境胁迫和激素信号诱导,通过蛋白和基因层面对逆境进行响应,JAZ蛋白在其中起到了桥梁的作用,并扮演了重要的角色。This study aims to clone the FmJAZ1 gene and clarify its response model under low temperature and NaCl stresses and its hormone-induced transcriptional expression characteristics.The FmJAZ1 gene of Fraxinus mandshurica Rupr.was obtained by gene cloning.The sequence was analyzed by bioinformatics software and phylogenetic tree was constructed.Spatiotemporal expression specificity of Fraxinus mandshurica Rupr.FmJAZ1 gene was analyzed.Samples of root,stem,leaf,bud,male flower,female flower and seed were taken in May,June,July,August,and September respectively for plants of Fraxinus mandshurica Rupr.under low temperature stress(4℃)and salt stress(NaCl solution),or treated with hormone signal induction of abscisic acid(ABA),gibberellin(GA3),auxin(IAA),jasmonate(JA),and salicylic acid(SA).The treated materials were then subjected to fluorescence quantitative analysis.The full length nucleotide sequence of 684 bp was cloned in this experiment.Bioinformatics software analysis showed that JZA1 gene had a complete open reading frame,encoding 227 amino acids.JAZ1 protein did not contain signal peptide,it was not a transmembrane protein,and it was an unstable hydrophilic protein.The spatial expression of FmJAZ1 gene was the highest in stem,and the temporal expression was the highest in August.The expression level of FmJAZ1 gene was higher at 6 h and 24 h after low temperature treatment,and higher 24 h after NaCl treatment,with the response to low temperature stress faster than that to salt stress.The expression level of FmJAZ1 gene changed significantly with time after the treatments of hormone signal induction,and the expression pattern of FmJAZ1 gene was analyzed for low temperature,NaCl stress and hormone induction treatments.The expression change of FmJAZ1 gene was 77.3 times higher than that of control group at 3 h after GA3 treatment.FmJAZ1 gene fully responded to abiotic stresses and hormone signal induction,with JAZ protein acting as a bridge and playing an important role in the responses at protein and gene levels

关 键 词：FmJAZ1 非生物胁迫 激素信号诱导 

分 类 号：S792.41[农业科学—林木遗传育种]