翡翠贻贝酶解液体外降压活性评价方法研究  

Study on the Method for Evaluating the Antihypertensive Activity of the Enzymatic Hydrolysate of Perna viridis in vitro

作　　者：安婷婷[1] 王鹤[1] 邓长春 林资政 AN Ting-ting;WANG He;DENG Chang-chun(Pharmaceutical Institute of Hainan Province,Haikou,Hainan 570311)

机构地区：[1]海南省药物研究所,海南海口570311

出　　处：《安徽农业科学》2020年第7期219-220,225,共3页Journal of Anhui Agricultural Sciences

基　　金：海南自然科学基金面上项目(817201)。

摘　　要：[目的]研究翡翠贻贝酶解液的体外降压活性,为后期开发药品和保健食品等提供前期的理论和试验基础。[方法]采用反向高效液相色谱法检测翡翠贻贝酶解液的体外降压活性。色谱柱:YMC C(18)柱(250 mm×4.6 mm,5μm);流动相:A为0.1%TFA乙腈,B为0.1%TFA水;测定波长228 nm;流速1 mL/min;进样量20μL。[结果]该方法使翡翠贻贝降血压肽、HHL和马尿酸较好地分离,在0.02~0.20 mg/mL线性关系良好(R^2=0.997 2),最低检出限为50 ng,定量限为200 ng,平均回收率为101.87%。[结论]该方法简便、快速、灵敏度高,可用于评价翡翠贻贝降血压肽的体外活性。[Objective]The research aimed to study the antihypertensive activity of the emerald mussel enzymatic hydrolysate in vitro,and to provide the theoretical and experimental basis for the early development of drugs and health foods.[Method]The RP-HPLC method was established for determining the activity of enzymatic hydrolysate of Perna viridis in vitro.It was analyzed by HPLC on a YMC C 18 colunm(250 mm×4.6 mm,5μm)and detected at the wavelength of 228 nm;mobile phase:A was 0.1%TFA acetonitrile,B was 0.1%TFA water;the velocity of flow was 1.0 mL/min and sample size was 20μL.[Result]This method could separate the enzymatic hydrolysate of Perna viridis,HHL and hippuric acid well.There was perfect linear correlation between 0.02-0.20 mg/mL(R^2=0.9972).The detection limit was 50 ng and the limit of quantitation was 200 ng.The average recovery rate was 101.87%.[Conclusion]The method is simple,rapid and sensitive.It can be used to evaluate the enzymatic hydrolysate of Perna viridis activity in vitro.

关 键 词：翡翠贻贝酶解液 反相高效液相色谱法 体外 降压活性 

分 类 号：TQ914.2[化学工程]