迪庆藏猪Nramp1基因第二内含子和第二外显子多态性位点遗传分析  

Genetic Analysis of the Polymorphism in the Second Intron and the Second Exon of Nramp1 Gene in Diqing Tibetan Pigs

作　　者：相德才 张斌 刘邵娜 杨仁灿 赵彦光 XIANG Decai;ZHANG Bin;LIU Shaona;YANG Rencan;ZHAO Yanguang(Yunnan Animal Science and Veterinary Institute,Kunming 650224,China;Yunnan Breeding Pig Performance Test Station,Kunming 650224,China;Yunnan Breeding Pig Quality Inspection and Test Center,Kunming 650224,China)

机构地区：[1]云南省畜牧兽医科学院,云南昆明650224 [2]云南省种猪性能测定站,云南昆明650224 [3]云南省种猪质量检验测试中心,云南昆明650224

出　　处：《养猪》2020年第2期60-62,共3页Swine Production

基　　金：云南省重点研发项目(2018BB003);云南省省级财政项目(20151105562255);云南省应用基础研究计划项目(2017FD055,2018FD001);云南省畜牧兽医科学院基础研究项目(2019RW008,2019RW012)。

摘　　要：以迪庆藏猪为研究对象,通过测序技术对Nramp1基因内含子2和外显子2进行多态性位点遗传分析。结果发现:迪庆藏猪在Nramp1基因内含子2上存在6个SNP位点,在外显子2上存在1个SNP;各SNPs均处于Hardy-Weinberg平衡,多态信息含量和杂合度均小于0.5。通过该研究了解了迪庆藏猪Nramp1基因的变异情况及群体遗传特征,为下一步开展抗病毒基因的遗传育种奠定了理论基础。In this study,Diqing Tibetan pigs was used as the research object to analyze the genetic polymorphism of intron 2 and exon 2 of Nramp1 gene by sequencing technology.The results showed that there were 6 SNPS on intron 2 and 1 SNP on exon 2 of Nramp1 gene.All SNPs were in Hardy-Weinberg equilibrium,the polymorphic information content and heterozygosity were less than 0.5.Through this study,the variation and population genetic characteristics of Nramp1 gene in Diqing Tibetan pigs were understood,which laid a theoretical foundation for the genetic breeding of antiviral gene in the next step.

关 键 词：迪庆藏猪 Nramp1基因 SNPS 

分 类 号：S813.1[农业科学—畜牧学]