蒙药苏格木勒-3汤对异丙肾上腺素诱导HL-1心肌细胞钙超载损伤的影响  

Effects of Mongolian Medicine Sugemule-3 Decoction (苏格木勒-3汤) on Isoproterenol-induced Calcium Overload Injury in HL-1 Cardiomyocytes

作　　者：王羽[1] 李凌儿 于丽君[1] 刘明洁[1] 郭欣 付瑶 魏成喜[1] WANG Yu;LI Ling'er;YU Lijun;LIU Mingjie;GUO Xin;FU Yao;WEI Chengxi(Inner Mongolia University for Nationalities,Tongliao,028000)

机构地区：[1]内蒙古民族大学,028000

出　　处：《中医杂志》2020年第6期524-527,共4页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81760780,81560590,8186140942);内蒙古自治区自然科学基金博士基金(2016BS0806);内蒙古自治区高等学校“青年科技英才”支持计划(NJYT-19-B14)。

摘　　要：目的探讨苏格木勒-3汤治疗心力衰竭的可能作用机制。方法 HL-1心肌细胞按每孔1×10~5个细胞接种于96孔板,常规培养24h后进行分组:空白对照组的不加任何处理的正常心肌细胞;模型组加入终浓度为25μg/ml的异丙肾上腺素每孔100μl处理24h;苏格木勒-3汤高、中、低剂量组加入终浓度为25μg/ml异丙肾上腺素每孔100μl处理24h后分别加入75、50、25μg/ml苏格木勒-3汤水提液每孔100μl处理24h;苏格木勒-3汤对照组加入75μg/ml苏格木勒-3汤水提液每孔100μl处理24h。每组5个复孔。MTT法检测各组HL-1心肌细胞存活率,荧光显微镜检测细胞中钙离子浓度,Western blotting法检测HL-1心肌细胞中肌浆网钙ATP酶2a (SERCA2a)蛋白表达。结果与空白对照组比较,模型组HL-1心肌细胞存活率、SERCA2a蛋白表达下降(P<0.01)。与模型组比较,苏格木勒-3汤高、中剂量组HL-1心肌细胞存活率增高,苏格木勒-3汤高、中、低剂量组HL-1心肌细胞SERCA2a蛋白表达量均增加(P<0.01)。荧光显微镜下显示,模型组HL-1心肌细胞内钙离子浓度的荧光强度增加;与模型组比较,苏格木勒-3汤中、高剂量组对HL-1心肌细胞内钙离子浓度的荧光强度逐渐下降。结论蒙药苏格木勒-3汤对HL-1心肌细胞钙超载具有抑制作用,其机制可能与提高心肌细胞中SERCA2a蛋白表达有关。Objective To explore the possible mechanism of Sugemule-3 Decoction(苏格木勒-3汤) in the treatment of heart failure. Methods HL-1 cardiomyocytes were inoculated into 96-well plates at 1×10~5 cells per well, and they were grouped after 24 h of routine culture. In blank control group, there were normal cardiomyocytes without any treatment. Model group was added with 100μl isoproterenol per well with a final concentration of 25μg/ml to process for 24 h. After being added with 100μl isoproterenol per well with a final concentration of 25μg/ml to process for 24 h, Sugemule-3 Decoction groups of high, medium and low doses were added with 75, 50, and 25μg/ml water extract of Sugemule-3 Decoction, respectively, to process for 24 h, with 100μl per well. There were 5 double wells in each group. MTT method was used to detect the survival rate of HL-1 cardiomyocytes in each group. Calcium ion concentration was detected by fluorescence microscope. Western blotting method was used to detect protein expression of sarcoplasmic reticulum calcium ATPase 2 a(SERCA2 a) in HL-1 cardiomyocytes. Results Compared with the blank control group, the survival rate of HL-1 cardiomyocytes and the expression of SERCA2 a protein in the model group were decreased(P<0.01). Compared with the model group, the survival rates of HL-1 cardiomyocytes in the high and medium dose groups of Sugemule-3 Decoction were increased, and the expressions of SERCA2 a protein in HL-1 cardiomyocytes of the high, medium and low dose groups of Sugemule-3 Decoction were increased(P<0.01). Fluorescence microscope showed that the fluorescence intensity of calcium ion concentration in HL-1 cardiomyocytes of the model group was increased;compared with the model group, the fluorescence intensities of calcium ion concentration in HL-1 cardiomyocytes in the medium and high dose groups of Sugemule-3 Decoction were decreased. Conclusion Mongolian medicine Sugemule-3 Decoction can inhibit calcium overload of HL-1 cardiomyocytes, and its mechanism may be related to in

关 键 词：心力衰竭 苏格木勒-3汤 心肌细胞 钙超载 肌浆网钙ATP酶2a 

分 类 号：R29[医药卫生—民族医学]