外源性硫化氢对高静止压力下兔血管平滑肌细胞增殖的影响  

Effect of hydrogen sulfide on the proliferation of rabbit vascular smooth muscle cell under high static pressure

作　　者：何天敏[1] 吴捷[1] 许昌声[2] 陈篇章 蔡方刚[1] He Tianmin;Wu Jie;Xu Changsheng;Chen Pianzhang;Cai Fanggang(Department of Vascular Surgery,the First Affiliated Hospital of Fujian Medical University,Fuzhou 350005,China;Fujian Institute of Hypertension,the First Affiliated Hospital of Fujian Medical University,Fuzhou 350005,China)

机构地区：[1]福建医科大学附属第一医院血管外科,福州350005 [2]福建医科大学附属第一医院高血压研究所,福州350005

出　　处：《中华普通外科杂志》2020年第3期232-235,共4页Chinese Journal of General Surgery

基　　金：福建省自然科学基金资助项目(2016J01536)。

摘　　要：目的观察外源性硫化氢对高静止压力下兔血管平滑肌细胞(VSMC)增殖的影响并探讨其可能分子机制。方法血管组织贴块法分离兔胸主动脉VSMC并于100 mmHg静止压力下培养,实验分为对照组,培养液含0.2%胎牛血清(FBS)但不含NaHS,10%FBS组及NaHS组(培养液含10%FBS且含50mmol/L NaHS)。应用5-溴脱氧尿嘧啶核苷法检测VSMC的增殖,并用Western-blotting法检测VSMC的内源性胱硫醚-γ-裂解酶(CSE)、钙调蛋白(Calmodulin,p-CaM/CaM)、CyclinD1蛋白水平。结果与10%FBS组相比,NaHS可显著抑制100 mmHg压力下兔VSMC的增殖(0.50±0.03比0.26±0.03,P<0.05)。相比于对照组,10%FBS组CSE蛋白表达水平显著降低(1.21±0.10比0.33±0.04,P<0.05),p-CaM、CylinD1二者表达显著增加(0.23±0.04比0.86±0.04与0.22±0.03比1.19±0.06,P<0.05)。与10%FBS组相比,NaHS组CSE蛋白表达水平显著增加(0.33±0.04比1.11±0.11,P<0.05),而p-CaM、CyclinD1蛋白表达水平均显著降低(0.86±0.04比0.26±0.05与1.19±0.06比0.51±0.03,P<0.05)。结论外源性硫化氢通过细胞内CSE/硫化氢信号通路抑制高静止压力下的兔VSMC增殖可能与p-CaM与CyclinD1表达抑制有关。Objective To observe the effect of exogenous hydrogen sulfide(H2S)on the proliferation of rabbit vascular smooth muscle cell(VSMC)under high static pressure.Methods Rabbit thoracic aorta VSMC were isolated and cultured under high static pressure(100mmHg)and divided into control group[cultured with 0.2%fetal bovine serum(FBS)and no NaHS]、10%FBS group(10%FBS and no NaHS)and NaHS group(10%FBS and 50mmol/L NaHS).VSMC proliferation was analyzed with BUDR.CSE,Calmodulin(p-CaM)and CyclinD1 protein levels of VSMC were measured by Western blotting.Results Compared with the 10%FBS group,NaHS inhibit the proliferation of rabbit VSMC significantly(0.50±0.03 vs.0.26±0.03,P<0.05).Compared with control group,CSE protein in the 10%FBS group decreased significantly(1.21±0.10 vs.0.33±0.04,P<0.05)and p-CaM and CyclinD1 protein increased significantly(0.23±0.04 vs.0.86±0.04 and 0.22±0.03 vs.1.19±0.06,P<0.05).Compared with the 10%FBS group,CSE protein in NaHS group increased significantly(0.33±0.04 vs.1.11±0.11,P<0.05),and the expression of p-CaM and CyclinD1 protein decreased significantly(0.86±0.04 vs.0.26±0.05 and 1.19±0.06 vs.0.51±0.03,P<0.05).Conclusion Exogenous hydrogen sulfide inhibits the proliferation of VSMC under high static pressure by the CSE/H2S pathway which related to the reduction of the expression of p-CaM and CyclinD1.

关 键 词：硫化氢 增殖 钙调蛋白 CYCLIND1 

分 类 号：R54[医药卫生—心血管疾病]