基于近红外光谱技术的复方大黄汤浓缩液同步快速检测  

Simultaneous and Rapid Detection of Concentrated Liquid of Compound Dahuang Decoction Based on Near Infrared Spectroscopy

作　　者：黄慧敏 张爱玲[2] 温伍玖 陶青 杨小妹[3] 蔡大宇 罗晓健[1,2] 何雁[1] HANG Hui-min;ZHANG Ai-ling;WEN Wu-jiu;TAO Qing;YANG Xiao-mei;CAI Da-yu;LUO Xiao-jian;HE Yan(Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China;National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medline,Nanchang 330006,China;Shenzhen Shekou People's Hospital,Shenzhen 518067,China;Truking Technology Co.Ltd.,Ningxiang 410600,China)

机构地区：[1]江西中医药大学,南昌330004 [2]中药固体制剂制造技术国家工程研究中心,南昌330006 [3]深圳市蛇口人民医院,广东深圳518067 [4]楚天科技股份有限公司,湖南宁乡410600

出　　处：《中国实验方剂学杂志》2020年第4期126-132,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家绿色制造系统集成项目(工信部节函(2016)562号);国家“重大新药创制”科技重大专项(2018ZX09201-010)。

摘　　要：目的:采用近红外光谱技术检测复方大黄汤浓缩过程中浓缩液密度、含固量、大黄酸和甘草酸质量浓度4个指标,建立该复方浓缩过程中多项指标的快速定量分析方法。方法:运用近红外光纤透射光谱法对复方大黄汤浓缩液进行快速测定,通过高效液相色谱法(HPLC)测定大黄酸和甘草酸的含量,选择51个样品进行内部交叉验证,采用偏最小二乘法回归分别建立近红外光谱与密度、含固量和指标成分含量之间的校正模型,应用采集的10个未知浓缩液样品进行外部验证预测。结果:复方大黄汤浓缩液近红外光谱与密度、含固量、大黄酸及甘草酸质量浓度的外部验证复相关系数(R2)分别为0.9959,0.9996,0.9970和0.9922,预测均方根误差(RMSEP)分别为2.50×10-3,0.17,7.57,67.10。结论:近红外光谱技术适用于复方大黄汤浓缩液的评价指标测定,且具有分析快速、简便、稳定可靠的特点。Objective:Near infrared spectroscopy was used to detect the concentration density(25℃),solid-containing content,rhein content and glycyrrhizic acid content of compound Dahuang decoction.Method:The concentrated liquid of compound Dahuang decoction was determined by near infrared optical fiber transmission spectrometry.The contents of rhein and glycyrrhizic acid were determined by HPLC.Fifty-one samples were used for internal cross-validation,and partial least square regression was used to establish correction models between near-infrared spectrum and density,solid-containing content,rhein content and glycyrrhizic acid content,respectively.Ten unknown concentrated liquid samples were collected for external validation and prediction.Result:The external validation complex correlation coefficients between near-infrared spectra and density,solidcontaining content,rhein content and glycyrrhizic acid content of the concentrated liquid of compound Dahuang decoction were 0.9959,0.9996,0.9970 and 0.9922,and the root mean square error of prediction(RMSEP)values were 2.50×10-3,0.17,7.57 and 67.10,respectively.Conclusion:The near infrared spectroscopy is suitable for the determination of evaluation indexes of the concentrated liquid index of compound Dahuang decoction,and has the characteristics of rapid,simple,stable and reliable.

关 键 词：近红外光谱 复方大黄汤 浓缩液 密度 含固量 大黄酸 甘草酸 

分 类 号：R22[医药卫生—中医基础理论] O657.33[医药卫生—中医学]