施氮量对刺五加幼苗抗氧化酶系统的影响  

Effect of Nitrogen Application on Antioxidant Enzyme System of Acanthopanax senticosus

作　　者：刘寒[1] 金司阳 杨立学[2] 王谦博[3] 郭盛磊[1] 王振月[1] LIU Han;JIN Siyang;YANG Li-xue;WANG Qian-bo;GUO Sheng-lei;WANG Zhen-yue(School of Pharmacy,Heilongjiang University of Chinese Medicine,Harbin 150040,China;School of Forestry,Northeast Forestry University,Harbin 150040,China;The First Affiliate Hospital of Guangdong Pharmaceutical University,Guangzhou 510080,China)

机构地区：[1]黑龙江中医药大学药学院,哈尔滨150040 [2]东北林业大学林学院,哈尔滨150040 [3]广东药科大学附属第一医院,广州510080

出　　处：《中国实验方剂学杂志》2020年第4期157-161,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点研发计划项目(2016YFC0500303);黑龙江省国家科技重大专项和重点研发项目省级项目(GX17C006);黑龙江中医药大学研究生创新科研项目(2018年)。

摘　　要：目的:该文通过研究不同施氮(N)量对刺五加幼苗生长发育及抗氧化酶的影响,筛选出适宜其生长发育的氮肥施用量。为刺五加人工栽培的科学施肥提供依据和指导。方法:采用单点单因素大田实验,以长势均一的1年生刺五加幼苗为研究样本,进行5个不同的施氮量处理,分别为N1组(30 g·m^-2),N2组(60 g·m^-2),N3组(90 g·m^-2),N4组(120 g·m^-2),N5组(150 g·m^-2),以及CK组(0 g·m^-2)。3个月后,收获时测定植株鲜重、根鲜重、叶鲜重、茎鲜重,干燥至恒重后测量植株干重、茎干重、叶干重以及根干重。采集植物新鲜叶片测量丙二醛(MDA)含量及过氧化氢酶(CAT),超氧化物歧化酶(SOD),过氧化物酶(POD),酸性磷酸酶(ACP),抗坏血酸过氧化物酶(APX)等抗氧化酶活力。结果:N4处理组最利于刺五加植株生物量的积累,该处理下刺五加幼苗产量最高,N3处理组的刺五加蛋白质含量最高,N3处理组各抗氧化酶活力均最低,表示此时植株所受环境胁迫影响最小。结论:刺五加幼苗对施氮量存在剂量效应关系,即低施氮量处理和高施氮量处理都会对刺五加造成逆境胁迫,且低施氮量环境下的抗氧化酶活力高于高施氮量,通过研究,得出适宜刺五加生长的施氮量为90~120 g·m^-2。Objective:To study the effect of different N application rates on the growth and development of Acanthopanax senticosus and the changes of antioxidant enzyme activity,and screen out the suitable amount of nitrogen fertilizer for its growth and development,in order to provide scientific evidence for rational fertilization of Acanthopanax senticosus in artificial cultivation.Method:By single-point,single-factor field experiment,the study samples were one-year-old seedlings of growing evenly A.senticosus.Five nitrogen application treatment groups were set up in the fields.They were N1(30 g·m^-2),N2(60 g·m^-2),N3(90 g·m^-2),N4(120 g·m^-2),N5(150 g·m^-2)and CK(0 g·m^-2)in the control group.Three months later,the raw weight of plant,root,leaf and stem were measured at harvest time.After drying to constant weight,plant dry weight,stem dry weight,leaf dry weight and root dry weight were measured.Fresh leaves of plants were collected to measure malondialdehyde(MDA)content and activities of catalase(CAT),superoxide dismutase(SOD),peroxidase(POD),acid phosphatase(ACP)and aseorbateperoxidase(APX)after harvesting seedlings.Result:The biomass of A.senticosus in group N4(120 g·m^-2)was the highest,the protein content in group N3(90 g·m^-2)was the highest,and the activity of all antioxidant enzymes in group N3(90 g·m^-2)was the lowest.Conclusion:There is a dose-effect relationship between seedlings and the nitrogen application rate.That is to say,low nitrogen application rate and high nitrogen application rate will cause stress on Acanthopanax senticosus,and the activity of antioxidant enzymes under low nitrogen application rate is higher than that under high nitrogen application rate.

关 键 词：刺五加 抗氧化酶 氮素 丙二醛 过氧化氢酶 超氧化物歧化酶 过氧化物酶 酸性磷酸酶 

分 类 号：R284.2[医药卫生—中药学] R289[医药卫生—中医学]