植物-土壤反馈对刺五加幼苗次生代谢产物的影响  

Effect of Plant-Soil Feedback on Secondary Metabolites of Acanthopanax senticosus Seedlings

作　　者：金司阳 刘寒[1] 杨立学[2] 王谦博[3] 徐明远 李俊萍 王振月[1] JIN Si-yang;LIU Han;YANG Li-xue;WANG Qian-bo;XU Ming-yuan;LI Jun-ping;WANG Zhen-yue(Heilongjiang University of Chinese Medicine,Harbin 150040,China;School of Forestry,Northeast Forestry University,Harbin 150040,China;The First Affiliated Hospital,School of Clinic Medicine of Guangdong Pharmaceutical University,Guangzhou 510080,China)

机构地区：[1]黑龙江中医药大学药学院,哈尔滨150040 [2]东北林业大学林学院,哈尔滨150040 [3]广东药科大学附属第一医院,广州510080

出　　处：《中国实验方剂学杂志》2020年第4期167-173,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点研发计划项目(2016YFC0500303);国家科技重大专项和重点研发项目省级项目(GX17C006);黑龙江中医药大学研究生创新科研项目(2018年)。

摘　　要：目的:研究植物-土壤反馈对刺五加幼苗根、茎、叶次生代谢产物的影响。方法:通过温室盆栽试验,分别对未种植过刺五加的土壤(1组),连续3年种植刺五加的土壤(2组)和多年种植刺五加的土壤(3组),分别种植刺五加1年生幼苗,并对其根、茎、叶的次生代谢产物进行分析。结果:L-苯丙氨酸,原儿茶酸,刺五加苷B,绿原酸,咖啡酸,刺五加苷E,异嗪皮啶,芦丁,金丝桃苷,槲皮素在多年生长刺五加土壤种植,对刺五加幼苗叶和根均有显著性差异,但在茎中绿原酸和刺五加苷E无显著性差异。其中刺五加苷E,异嗪皮啶,芦丁和金丝桃苷在多年生刺五加土壤种植的幼苗叶中未检出。在刺五加幼苗的根中,多数次生代谢产物呈现正反馈;在刺五加幼苗的茎中,咖啡酸,刺五加苷E,金丝桃苷,槲皮素呈现负反馈;在刺五加幼苗的叶中多数次生代谢产物呈现正反馈。结论:植物和土壤在刺五加幼苗生长过程不同部位呈现出不同的反馈情况,整体而言,未种植过刺五加的土壤对刺五加幼苗的次生代谢产物更具优势。研究结果为阐述植物-土壤反馈对刺五加的影响提供研究基础,并为人工栽培刺五加提供了理论依据和技术支持。Objective: To investigate the effects of plant-soil feedback on secondary metabolites in roots,stems and leaves of Acanthopanax senticosus seedlings. Method: One-year-old seedlings of A. senticosus were planted in the soil where no A. senticosus had been planted before(group 1),soil where A. senticosus had been planted for 3 years(group 2),and soil where A. senticosus had been planted for many years in the greenhouse pot experiment,and the secondary metabolites of its roots, stems and leaves were then analyzed. Result: LPhenylalanine, 3, 4-dihydroxybenzoic acid, syringin, chlorogenic acid, caffeic acid, eleutheroside E,isofraxidin,rutin, hyperoside, and quercetin had significant differences in leaves and roots of A. senticosus seedlings in the soil of group 3,but there was no significant difference in chlorogenic acid and eleutheroside E in stem. Eleutheroside E,isofraxidin,rutin and hyperoside were not detected in the leaves of seedlings planted in group 3. Most of the secondary metabolites in the roots of A. senticosus seedlings showed positive feedback,while in the stems of Acanthopanax senticosus seedlings, caffeine, A. senticosus glycosides, hypericin and quercetin showed negative feedback,and most of the secondary metabolites in the leaves of A. senticosus seedlings showed positive feedback. Conclusion: The plant and soil showed different feedback in different parts of the growth process of A. senticosus seedlings,and the soil where A. senticosus had not been planted was more advantageous to the secondary metabolites of A. senticosus seedlings. The results of the study provide a basis for the study of the effect of plant-soil feedback on the A. senticosus,and provide the theoretical basis and technical support for the artificial cultivation of A. senticosus.

关 键 词：刺五加 次生代谢 植物-土壤反馈 幼苗 根 茎 叶 

分 类 号：R284.2[医药卫生—中药学] R289[医药卫生—中医学]