人脐血间充质干细胞移植修复骨髓造血损伤  

Transplantation of human umbilical cord blood mesenchymal stem cells in the repair of hematopoietic injury in bone marrow

作　　者：高坤莉 邢宏运 卞铁荣 韩丽英 Gao Kunli;Xing Hongyun;Bian Tierong;Han Liying(Department of Hematology,the Affiliated Hospital of Southwest Medical University,Luzhou 646000,Sichuan Province,China;Department of General Practice,the Affiliated Hospital of Southwest Medical University,Luzhou 646000,Sichuan Province,China;Medical Experiment Center,the Affiliated Hospital of Southwest Medical University,Luzhou 646000,Sichuan Province,China)

机构地区：[1]西南医科大学附属医院血液内科,四川省泸州市646000 [2]西南医科大学附属医院全科医学科,四川省泸州市646000 [3]西南医科大学附属医院医学实验中心,四川省泸州市646000

出　　处：《中国组织工程研究》2020年第25期3966-3973,共8页Chinese Journal of Tissue Engineering Research

基　　金：西南医科大学附属医院重点课题(2014ZD-002),项目负责人:邢宏运;四川省教育厅重点课题(16ZA01),项目负责人:邢宏运。

摘　　要：背景:大多数研究间充质干细胞体外培养对造血干细胞的增殖作用和骨髓间充质干细胞移植可降低辐照引起的造血细胞死亡,增加骨髓细胞存活,修复造血功能,而少有研究人脐血间充质干细胞移植对骨髓造血损伤的修复。目的:探讨人脐血间充质干细胞对骨髓造血微环境的修复情况。方法:选用雄性BALB/c小鼠随机分为3组,实验组和对照组小鼠进行总剂量为6 Gy的X射线全身照射,建立骨髓造血损伤模型,正常组为未经处理的正常小鼠。实验组小鼠照射当天经尾静脉输入CM-DiL标记的人脐血间充质干细胞5×10^6/只(0.2 mL),对照组和正常组经尾静脉输入生理盐水0.2 mL,移植后第1,5,7,14,21天观察外周血血象恢复情况和骨髓造血微环境修复情况。结果与结论:①外周血常规:移植后第1,5,7天,实验组和对照组小鼠与正常组小鼠比较,白细胞、血小板、红细胞计数及血红蛋白浓度进行性下降,第7天下降最为明显,移植后第14天三系较前有所恢复,移植后第21天基本恢复正常,与实验组相比,对照组三系下降更为明显,移植后第14天实验组较对照组恢复快;②骨髓涂片情况:移植后第1,5,7,14天实验组及对照组小鼠骨髓出现造血功能抑制,以第7天最为明显,移植后第14天骨髓增生较前有所恢复,实验组优于对照组;移植后第21天实验组及对照组小鼠骨髓造血功能恢复,与正常组相比无差异;③骨髓病理切片情况:移植后第1,5,7,14天实验组及对照组小鼠骨髓出现造血功能抑制;移植后第14天实验组及对照组小鼠的骨髓造血功能较前开始恢复,实验组小鼠的骨髓增生情况优于对照组小鼠,移植后第21天实验组及对照组小鼠骨髓增生情况与正常组比较无差异;④结果表明,人脐血间充质干细胞对骨髓造血功能恢复均有明显促进作用。BACKGROUND: A large number of studies mainly concern the proliferation effect of mesenchymal stem cells on hematopoietic stem cells in vitro and that bone marrow mesenchymal stem cell transplantation can reduce the death of hematopoietic cells caused by irradiation, increase the survival of bone marrow cells and repair hematopoiesis, while few of them investigate the repair of human umbilical cord blood mesenchymal stem cells transplantation on bone marrow hematopoiesis injury. OBJECTIVE: To explore the repair of hematopoietic microenvironment of bone marrow by human umbilical cord blood mesenchymal stem cells. METHODS: Male BALB/c mice were randomly divided into three groups. The mice in experimental group and control group were irradiated with total dose of 6-Gy X-ray to establish a mouse model of bone marrow hematopoietic injury. The normal group contained untreated normal mice. In the experimental group, CM-DiL labeled human umbilical cord blood mesenchymal stem cells were injected into the tail vein of each mouse at 5×10^6 (0.2 mL). The control group and the normal group received normal saline 0.2 mL through the tail vein. The peripheral blood hematology and bone marrow hematopoietic microenvironment repair were observed at 1, 5, 7, 14 and 21 days after cell transplantation. RESULTS AND CONCLUSION: Peripheral blood condition: At 1, 5 and 7 days after transplantation, the leucocyte, platelet, erythrocyte count and hemoglobin concentration in the experimental group and control group decreased progressively compared with the normal group. The most obvious decrease occurred on day 7. The trilineage recovered on day 14 after transplantation, basically returned to normal on day 21 after transplantation. Compared with the experimental group, the decrease of the trilineage in the control group was more obvious. The recovery was obvious faster in the experimental group than in the control group on day 14 after transplantation. Bone marrow smears: Bone marrow smears showed that the hematopoietic function was inhibite

关 键 词：人脐血间充质干细胞 骨髓造血 造血功能 骨髓增生 

分 类 号：R459.9[医药卫生—治疗学] R394.2[医药卫生—临床医学]