细胞力学机械刺激对牙髓干细胞的影响  

Effect of mechanical stimulation on dental pulp stem cells

作　　者：李峻青 吴家媛 Li Junqing;Wu Jiayuan(Zunyi Medical University,Zunyi 563000,Guizhou Province,China;Stomatological Hospital Affiliated to Zunyi Medical University,Zunyi 563000,Guizhou Province,China;Special Key Laboratory of Oral Diseases Research of Universities in Guizhou Province,Zunyi 563000,Guizhou Province,China)

机构地区：[1]遵义医科大学,贵州省遵义市563000 [2]遵义医科大学附属口腔医院,贵州省遵义市563000 [3]贵州省高等学校口腔疾病研究特色重点实验室,贵州省遵义市563000

出　　处：《中国组织工程研究》2020年第25期4054-4059,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81460102),项目负责人:吴家媛;贵州省科技厅项目(黔科合J字LKZ[2013]11号),项目负责人:吴家媛;贵州省高校优秀科技创新人才支持计划(合同号:KYJF(2014-8)),项目负责人:吴家媛;遵义市优秀青年科技创新人才培养项目(遵优青科【2018】8号),项目负责人:吴家媛。

摘　　要：背景:力学刺激对于机体内很多器官、组织的发育和损伤修复发挥重要的调控作用。除生物化学因素外,细胞力学等机械因素越来越被认为是影响牙髓干细胞行为和功能的关键调节因素。目的:综述细胞力学刺激对牙髓干细胞生物学行为的作用及影响。方法:通过检索PubMed、Embase、Medline、CNKI数据库,分别以“牙髓干细胞,机械压力,机械张力,剪切力,压应力,细胞增殖,成骨分化”为中文检索词和“human dental pulp stem cells(hDPSCs),mechanical strain,mechanical stretch,mechanical tension,shear stress,cell proliferation,osteogenesis differentiation”为英文检索词进行检索,选取与细胞力学机械刺激参与影响牙髓干细胞增殖、分化的56篇文献进行综述。结果与结论:细胞力学机械刺激是影响细胞增殖、分化、蛋白表达和凋亡的重要生物学因素。牙髓干细胞是牙髓组织中的间充质干细胞,其生物学行为也受到细胞力学机械刺激的影响。细胞力学刺激参与牙髓干细胞的增殖、成牙/成骨分化,并且当牙本质受到流体流动力作用时,会激活其机械感受器来调节并维持牙齿的完整性。介导牙髓干细胞生物学行为的信号通路包括MAPK、Wnt、Akt及BMP-7、Nrf2/HO-1等,通过调控这些信号通路进而对牙髓干细胞增殖及成牙/成骨分化发挥不同程度的促进及抑制作用。BACKGROUND:Mechanical stimulation plays a necessary regulatory role in developing and repairing many organs and tissues in the human body.Except for biochemical factors,mechanical factors are considered as key regulatory factors that affect the behavior and function of dental pulp stem cells.OBJECTIVE:To review the role and effect of cellular mechanical stimulation on the biological behavior of dental pulp stem cells.METHODS:PubMed,Embase,Medline and CNKI databases were searched for relevant literatures using the keywords of“human dental pulp stem cells(hDPSCs),mechanical strain,mechanical stretch,mechanical tension,shear stress,cell proliferation,osteogenesis differentiation”in English and Chinese,respectively.Fifty-six articles were finally eligible for review,which were closely related to the proliferation and differentiation of dental pulp stem cells under cellular mechanical stimulation.RESULTS AND CONCLUSION:Cellular mechanical stimulation is an important biological factor affecting cell proliferation,differentiation,apoptosis and protein expression.Dental pulp stem cells are mesenchymal stem cells derived from the dental pulp tissue,and their biological behaviors are also affected by cellular mechanical stimulation.Cellular mechanical stimulation is involved in the proliferation,odontogenesis/osteogenesis of dental pulp stem cells.When the dentin is subjected to fluid flow forces,mechanoreceptors are activated to regulate and maintain the integrity of tooth structure.Signal pathways that mediate the biological behavior of dental pulp stem cells include MAPK,Wnt,Akt,BMP-7,and Nrf2/HO-1,which are involved in promoting and inhibiting the proliferation and odontogenesis/osteogenesis of dental pulp stem cells to varying degrees.

关 键 词：牙髓干细胞 细胞增殖 成骨分化 牙源性牙本质干细胞 力学性能 细胞力学 生物学行为 牙再生组织工程 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]