抗菌核病甘蓝型油菜种质的筛选与鉴定  

Screening and Identification of Sclerotinia-resistantGermplasm of Brassica napus L.

作　　者：雷蕾 梁龙兵 秦信蓉 喻时周 徐静[1] 陈丽 侯燕 杜才富[1,3] LEI Lei;LIANG Longbing;QIN Xinrong;YU Shizhou;XU Jing;CHEN Li;HOU Yan;DU Caifu(Guizhou Rapeseed Institute,Guizhou Academy of Agricultural Sciences,Guiyang 550008,China;Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture,Wuhan Hubei 4300662,China;Guizhou Hemufu Seed Limited Company,Guiyang 550008,China)

机构地区：[1]贵州省农业科学院油菜研究所,贵阳550008 [2]农业部油料作物生物学与遗传育种重点实验室,湖北武汉430062 [3]贵州禾睦福种子有限公司,贵阳550008

出　　处：《种子》2020年第3期29-33,共5页Seed

基　　金：国家转基因重大专项“长江上游转基因转基因油菜新品种培育”(2018 ZX 08020001-007);省科技支撑计划“油菜特高含油量种质创新及应用”(黔科合支撑[2018]2347号);农业部油料作物生物学与遗传育种重点实验室开放课题资助项目(2017008);贵州省科研机构服务企业行动计划项目“油菜育繁推技术创新与服务”(黔科合服企[2015]4001号);贵州省高层次创新型人才项目(黔科合人才[2015]4018号);中央引导地地方科技发展专项资金项目任务书“贵州省油菜商业化育种技术平台建设”(黔科中引地[2016]4002号)。

摘　　要：菌核病是由核盘菌引起的常年严重危害油菜生产的真菌性病害。通过收集贵州油菜种植区田间菌核,对其进行培养繁殖获得大量核盘菌;利用繁殖的菌核对2 000份甘蓝型油菜恢复系种质资源进行连续2年田间鉴定,从中筛选出51份抗菌核病恢复系材料,其中39份材料的菌核病发病率、病情指数和相对发病指数均低于抗性对照中油821。结合油菜种子品质分析,结果显示,种子芥酸含量与油菜菌核病抗性成正相关关系。Rapeseed is an important cash crop in Guizhou province,and stalk break caused by sclerotinia is a fungal disease which seriously damages rapeseed production every year.In this study,a large number of sclerotinia sclerotiorum were obtained by culturing of sclerotium collecting from wild field.There were nearly 2000 restorer germplasms of Brassica napus L.were inoculated with the collected sclerotium and the field trial was carried out continuously for two years.Fifty-one sclerotium-resistant restorer lines were screened out,and the incidence rate,disease index and relative disease index of sclerotinia in 39 samples were lower than those of the resistant control Zhongyou 821.Combined with the analysis of rape seed quality,the results showed that the content of erucic acid was positively correlated with the resistance to sclerotia disease.

关 键 词：甘蓝型油菜 菌核病 抗病性 恢复系 种子品质 

分 类 号：S565.4[农业科学—作物学]