Fluorescence Signal Amplification Strategies Based on DNA Nanotechnology for miRNA Detection  

作　　者：LI Tao DUAN Ruilin DU AN Zhijuan HUANG Fujian XIA Fan 

机构地区：[1]Engineering Research Center ofNano-geomaterials,Ministry of Education,Faculty of Materials Science and Chemistry,China University of Geosciences,Wuhan 430074,R R.China

出　　处：《Chemical Research in Chinese Universities》2020年第2期194-202,共9页高等学校化学研究（英文版）

基　　金：Supported by the National Key R&D Program of China(No.2017YFA0208000);the National Natural Science Foundation of China(Nos.21974127,21525523,21722507,21874121,21974128);the Natural Science Foundation of Zhejiang Province,China(No.LY20B050001).

摘　　要：In this review,the most recent progresses in the field of fluorescence signal amplification strategies based on DNA nanotechnology for miRNA are summarized.The types of signal amplification are given and the principles of amplification strategies are explained,including rolling circle amplification(RCA),catalytic hairpin assembly(CHA),hybridization chain reaction(HCR)and DNA walker.Subsequently,the application of these signal amplification methods in biosensing and bioimaging are covered and described.Finally,the challenges and the outlook of fluorescence signal amplification methods for miRNA detection are briefly commented.

关 键 词：Signal AMPLIFICATION DNA NANOTECHNOLOGY FLUORESCENCE miRNA detection BIOSENSING and BIOIMAGING 

分 类 号：TB383[一般工业技术—材料科学与工程]