Ultrasensitive Detection of Hepatitis C Virus DNA Subtypes Based on Cucurbituril and Graphene Oxide Nano-composite  

作　　者：JIANG Ping LI Yifei JU Ting CHENG Wenbo XU Jianhua HAN Kun 

机构地区：[1]University of Science and Technology of China,Hefei 230027,P.R.China [2]C/LS Key Lab of Bio-medical Diagnostics,Suzhou Institute of Biomedical Engineering and Technology,Chinese Academy of Sciences,Suzhou 215163,P.R.China [3]University of Chinese Academy of Sciences,Beijing 100049,P.R China [4]Institute of Optics and Electronics,Chengdu 610209,P.R.China [5]Laboratory of Oncology Science and Molecular Biology,Shunde Hospital of Guangzhou University of Chinese Medicine,Foshan 528333,P.R.China

出　　处：《Chemical Research in Chinese Universities》2020年第2期307-312,共6页高等学校化学研究（英文版）

基　　金：Supported by the National Key R&D Program of China(No.2017YFC0108505);the Youth Innovation Promotion Association of China(No.2015261);the National Natural Science Foundation of China(No.31500805);the“333 High-level Talents Training Project of Jiangsu Province”,China;the Science and Technology Program of Suzhou,China(No.SYG201508).

摘　　要：Infectious of hepatitis C viruses(HCVs)lead to hepatic fibrosis,cirrhosis even hepatoma.Developing rapid and sensitive diagnostic method for HCV is of great importance.Based on the host-and-guest interaction between cucurbit[7]uril(CB[7])and methylene blue(MB),a CB[7]-graphene nano-composite(CB[7]-N3-GO)is raised for the electrochemical detection of HCV DNA.The method is able to linearly detect the HCV nucleic acid in the range of 0.2—10 nmol/L with detection limit as low as 160.4 pmol/L.The proposed detection strategy is able to discriminate the lb and 6k subtypes of HCV and has a prospective potential in the blood screen for HCV in clinical diagnosis.

关 键 词：Hepatitis C virus uril Graphene NANO-COMPOSITE Assay 

分 类 号：TQ127.11[化学工程—无机化工]