狂犬病病毒HEP-Flury M基因重排在小鼠致病性和保护性研究  

Pathogenicity and protection in mice of rearranging the M gene of RABV HEP-Flury

作　　者：梅明珠 杨先锋 龙腾 张琼[2] 赵静[2] 田钦 彭娇娇[2] 罗均[2] 姜贺 林颖仪[2] 林志雄 郭霄峰[2] MEI Ming-zhu;YANG Xian-feng;LONG Teng;ZHANG Qiong;ZHAO Jing;TIAN Qin;PENG Jiao-jiao;LUO Jun;JIANG He;LIN Ying-yi;LIN Zhi-xiong;GUO Xiao-feng(Guangzhou Custom Technology Center,Guangzhou 510623,China;College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]广州海关技术中心,广州510623 [2]华南农业大学兽医学院,广州510642

出　　处：《中国人兽共患病学报》2020年第3期173-177,共5页Chinese Journal of Zoonoses

基　　金：“十三五”国家重点专项(No.2016YFD0500400);国家自然科学基金(No.31772742);国家重大科学仪器设备开发专项(No.2012YQ09019705)联合资助。

摘　　要：目的了解M基因重排对病毒致病性和免疫保护性的影响,从而为狂犬病疫苗的开发提供更适合的候选株。方法将病毒rHEP-Flury和M基因重排株颅内接种1~3 d龄的乳鼠,测定病毒在乳鼠上的半数致死量(LD 50)。将rHEP-Flury和M基因重排株以103、104和105 FFU免疫接种6~8周龄的成鼠,然后用标准攻毒株CVS-24进行颅内感染小鼠,测定小鼠的存活率。同时用ELISA方法检测小鼠血清抗体水平。结果狂犬病病毒HEP-Flury的M基因重排后,病毒对乳鼠的毒力随着M基因位置远离病毒启动子位置增强,即M2<rHEP-Flury<M4。当接种剂量为104和105FFU时,M基因重排对病毒在成鼠上的保护性没有统计学意义影响;当接种剂量为103FFU时,M基因重排病毒对小鼠的保护性明显高于rHEP-Flury。结论狂犬病病毒HEP-Flury的M基因重排能够降低病毒对乳鼠的毒力,提高对小鼠的保护性,为狂犬病疫苗开发奠定基础。In order to help rabies vaccine candidate construction,the lethality and immunological protection between rHEP-Flury and M gene rearranged viruses were compared and analyzed.Lethality in suckling mice was performed by intracranial inoculating rHEP-Flury or M gene rearranged viruses,then LD 50 was calculated.Protection of mice was performed by immunized once intramuscularly with different doses of rHEP-Flury or M gene rearranged viruses,then mice were challenged IC with CVS-24 and survivor numbers were evaluated.At the same time,the antibody level was assessed by ELISA.The lethality in suckling mice increased as the M gene was moved successively away from promoter proximal position to successive positions down the viral genome,that is M2<rHEP-Flury<M4.Protection of mice showed that the percent survival at the dose of 104 and 105FFU has no significant difference,however,at the dose of 103FFU,the percent survival of M gene rearranged viruses was clearly higher than that of rHEP-Flury.M gene rearrange ment of HEP-Flury could decrease the viral lethality and increase the protection,which is benefit for the choose of rabies vaccine candidate.

关 键 词：HEP-Flury 狂犬病 基因重排 基质蛋白 

分 类 号：R373.9[医药卫生—病原生物学]