益肺温阳化浊汤对Aβ诱导的AD大鼠海马Aβ含量及凋亡因子的影响  

Effects of Yifeiwenyanghuazhuo Decoction on the AβContent and Apoptosis Factors in Hippocampus of AD Rats Induced by Aβ

作　　者：王晋平[1] 唐农[2] 吴林[2] 古联[1] 邢远[1] 陈炜[2] 李景玉[1] 程永芳 

机构地区：[1]广西中医药大学第一附属医院,广西南宁530023 [2]广西中医药大学中医基础研究重点实验室,广西南宁530001

出　　处：《大众科技》2020年第1期50-53,共4页Popular Science & Technology

基　　金：广西自然科学基金(2016GXNSFAA380170);广西自然科学基金联合项目(2018GXNSFAA294089);广西教育厅中青年教师基础能力提升项目(2017KY0318,J17099);广西中医基础研究重点实验室项目(KJT1701206,17-259-49-06);广西医疗卫生适宜技术开发与推广应用项目(S2018030);广西中医临床研究重点实验室项目(J16062)。

摘　　要：目的:探讨中药复方益肺温阳化浊汤对Aβ诱导的AD大鼠模型海马Aβ异常沉积与神经元凋亡的关系及Caspase-9、Caspase-3、Bcl-2、Bax凋亡相关因子的影响。方法:采用MorriS水迷宫测试各组大鼠行为学,流式细胞仪检测神经细胞凋亡率,Western Blot检测各组Caspase-9、Caspase-3、Bcl-2、Bax的表达;观察益肺温阳化浊汤低、中、高剂量对Aβ诱导的AD大鼠海马区Aβ含量及凋亡因子的影响。结果:模型大鼠经益肺温阳化浊汤处理后,海马区Aβ含量明显降低,神经元凋亡减轻,抑制Caspase-9、Caspase-3、Bax表达,上调抗凋亡蛋白Bcl-2的表达,并呈一定的剂量依赖性。结论:益肺温阳化浊汤可能通过促进Aβ诱导的AD大鼠海马区神经细胞中Aβ的清除,减轻Aβ的神经毒性,从而抑制神经元的凋亡,起到保护神经元的作用。Objective:To investigate the relationship between Aβabnormal deposition and neuronal apoptosis in Aβ-induced model hippocampus and the effects of TCM compound Yifeiwenyanghuazhou decoction on apoptosis-related factors of Caspase-9 Caspase-3,Bcl-2 and Bax apoptosis.Methods:MothriS water maze was used to test the behavior of each group of rats,flow cytometry was used to detect the apoptosis of nerve cells,and Western Blot detected the expression of Caspase-9,Caspase-3,Bcl-2,and Bax in each group;To observe the effect of low,medium and high dose of Yifeiwenyang chemical turbid decoction on the Aβcontent and apoptosis factor in the hippocampus of AD rats induced by Aβ.Results:After the treatment of Yiyangwenyangzhuo decoction,the content of Aβ in the hippocampus was significantly reduced,the apoptosis of neurons was reduced,the expression of Caspase-9,Caspase-3,Bax was inhibited,and the expression of the antiapoptosis protein Bcl-2 was increased,and showed a certain dose dependence.Conclusion:Yifeiwenyanghuazhuo decoction may reduce the neurotoxicity of Aβ by promoting the removal of Aβ in nerve cells in the hippocampus of AD rats induced by Aβ,reduce the neurotoxicity of aβ,thus inhibit the apoptosis of neurons,and play a role in protecting neurons.

关 键 词：阿尔茨海默病 益肺温阳化浊方 Β淀粉样蛋白 细胞凋亡 

分 类 号：R285[医药卫生—中药学]