对马铃薯类受体激酶CRK基因家族的鉴定及响应病原真菌信号的表达分析  

Genome wide identification and expression analysis of CRK gene family in response to fungal pathogen signals in potato

作　　者：张卫娜 范艳玲 康益晨 杨昕宇 石铭福 要凯 赵章平 张俊莲 秦舒浩 ZHANG Wei-Na;FAN Yan-Ling;KANG Yi-Chen;YANG Xin-Yu;SHI Ming-Fu;YAO Kai;ZHAO Zhang-Ping;ZHANG Jun-Lian;QIN Shu-Hao(College of Horticulture,Gansu Agricultural University,Lanzhou 730070,Gansu,China)

机构地区：[1]甘肃农业大学园艺学院,甘肃兰州730070

出　　处：《作物学报》2020年第5期680-689,共10页Acta Agronomica Sinica

基　　金：甘肃农业大学园艺学科建设基金项目(GAU-XKJS-2018-225);国家自然科学基金项目(31260311);甘肃省自然科学基金项目(1606RJZA034);中国博士后科学基金项目(2012M512042,2014T70942);国家现代农业产业技术体系(马铃薯)建设专项(CARS-09-P14)资助。

摘　　要：富含半胱氨酸的类受体激酶(cysteine-rich receptor-like kinase,CRK)在植物生长发育和环境适应过程中发挥重要的作用。本研究鉴定了马铃薯CRK(StCRK)家族成员,并对其理化性状、进化特征、亚细胞定位、染色体位置和表达模式进行分析。鉴定获得8个StCRKs,其氨基酸序列大小为459~686 aa,分子量介于50.75~77.50 kD,等电点介于5.84~8.75,主要位于质膜。进化分析将来自马铃薯、拟南芥、香蕉、苹果、水稻、番茄和棉花的CRKs分为9个亚组,2号、3号和5号染色体上的StCRKs分布于亚组I(6个成员)和VI(2个成员);存在2个串联重复基因簇,包含4个成员。StCRKs启动子区域存在多种顺式调控元件,主要响应激素、低温、防卫和逆境等信号。接种晚疫病菌(Phytophthora infestans,Pi)和干腐病菌(Fusarium sulphureum,Fs)后,分别发现8个和6个StCRKs为差异表达。其中,StCRK4和StCRK8响应Pi和Fs信号,在接种以上2种病原菌后,表达量上调8倍以上,推测其响应多个真菌信号,可能在马铃薯对真菌病害的广谱抗性中起重要作用,可作为进一步抗病研究和功能分析的候选基因。Cysteine-rich receptor-like kinase(CRK)plays an important role in plant growth and environmental adaptation.In this study,potato CRK(StCRK)family members were identified,and their physical and chemical characteristics,evolutionary characteristics,subcellular location,chromosome location and expression patterns were analyzed.Eight StCRK members were identified,with amino acid size from 459 to 686 aa,molecular weight of 50.75-77.50 kD,and isoelectric point of 5.84-8.75.StCRKs were mainly located on plasma membrane.CRKs from potato(Solanum tuberosum),apple(Malus pumila Mill.),Thale Cress(Arabidopsis thaliana),rice(Oryza sativa),cotton(G.hirsutum),banana(Musa acuminata),and tomato(Solanum lycopersicum)could be divided into nine subgroups,and StCRKs were belonged to subgroups I(6 members)and VI(2 members).Moreover,StCRKs distributed on chromosomes 2,3,and 5,contained two tandem repeat gene clusters,including four members.There were many cis-regulated elements in the StCRKs promoter region,which mainly respond to hormones,low temperature,defense and stress signals.After inoculating Phytophthora infestans(Pi)and Fusarium sulphureum(Fs),eight and six StCRKs gene differentially expressed,among them,StCRK4 and StCRK8 had the expression levels by more than eight times.It is speculated that they may respond to multiple fungal signals,and play an important role in potato's broad-spectrum resistance to fungal diseases,and can be used as candidate genes for further needed on disease resistance and functional analysis.

关 键 词：CRK 顺式表达元件 生物信息学分析 硫色镰刀菌 马铃薯晚疫病菌 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]