谷子PEPC基因的鉴定及其对非生物逆境的响应特性  

Identification of PEPC genes from foxtail millet and its response to abiotic stress

作　　者：赵晋锋[1] 杜艳伟[1] 王高鸿[1] 李颜方[1] 赵根有 王振华[1] 王玉文[1] 余爱丽[1] ZHAO Jin-Feng;DU Yan-Wei;WANG Gao-Hong;LI Yan-Fang;ZHAO Gen-You;WANG Zhen-Hua;WANG Yu-Wen;YU Ai-Li(Millet Research Institute,Shanxi Academy of Agricultural Sciences/Shanxi Key Laboratory of Genetic Resources and Breeding in Minor Crops,Changzhi 046011,Shanxi,China)

机构地区：[1]山西省农业科学院谷子研究所/特色杂粮种质资源发掘与育种山西省重点实验室,山西长治046011

出　　处：《作物学报》2020年第5期700-711,共12页Acta Agronomica Sinica

基　　金：山西省农业科学院项目(YGJPY2009,YGG17021,YCX2019T05);国家现代农业产业技术体系建设专项(CARS-06-13.5-A23);国家农业环境数据中心观测检测任务(ZX03S0410)项目资助。

摘　　要：磷酸烯醇式丙酮酸羧化酶(phosphoenolpyruvate carboxylase,PEPC)是C4植物光合作用关键酶,并在植物多种代谢途径及逆境信号应答过程中起重要作用。本研究通过序列比对,从谷子基因组中筛选出6个SiPEPC候选基因。SiPEPC蛋白特征参数相似度很高,序列非常保守,都含有PEPC基因特征功能域PEPcase Motif。SiPEPC成员主要被定位在细胞质、细胞核和线粒体。在SiPEPC成员启动子序列中含大量有光、激素、逆境以及其他生长调控相关的顺式应答元件。苗期逆境qRT-PCR表达谱分析表明,5个SiPEPC基因(SiPEPC1、SiPEPC2、SiPEPC3、SiPEPC5、SiPEPC6)不同程度受ABA、PEG、高盐和低温诱导表达,表明其参与了苗期对非生物逆境的响应。5个SiPEPC基因表达量在正常生长条件下随着谷子的生长呈增强趋势,且在不同生育时期干旱胁迫下明显增加,表明其参与了拔节、抽穗、灌浆期的干旱胁迫应答。拔节期弱光可诱导5个SiPEPC基因的表达,而在拔节期中等强度光照以及抽穗期和灌浆期的中等光照和弱光照下表达量均急剧降低,揭示光照强度严重影响SiPEPC基因的表达。Phosphoenolpyruvate carboxylase(PEPC)is a key enzyme in photosynthesis of C4 plants and plays an important role in a variety of metabolic and stress pathways.In this study,we identified six candidate PEPC genes from foxtail millet genome via sequence alignment.The characteristic parameters of all SiPEPC protein were very similar and the sequences were very conservative.All SiPEPC genes contained the PEPcase motif,which is the characteristic domain of PEPC gene.SiPEPCs were localized in cytoplasm,nucleus and mitochondrion.Promoter analysis identified a variety of light,hormonal,stress,and other growth-related cis-elements in the promoter sequences of SiPEPC members.The qRT-PCR expression profiles showed that the five SiPEPC genes(SiPEPC1,SiPEPC2,SiPEPC3,SiPEPC5,SiPEPC6)were induced by ABA,PEG,high salt and low temperature at seedling stage,indicating that five SiPEPC genes ate involved in abiotic signaling pathway at the seedling stage.The expression of five SiPEPC genes increased with the growth of foxtail millet under normal growth conditions,and increased significantly under drought stress at different growth stages,indicating that five SiPEPCs are involved in drought stress response at jointing,heading and filling stages.The weak light at jointing stage could induce the expression of five SiPEPC genes,while the expression level decreased sharply under moderate light intensity at jointing stage,moderate and weak light intensity at heading and filling stages,showing that light intensity seriously affects the expression of SiPEPC genes.

关 键 词：谷子 磷酸烯醇式丙酮酸羧化酶 非生物逆境 表达分析 

分 类 号：S515[农业科学—作物学]