高脂饲喂结合力竭游泳的气虚血瘀证动物模型的构建及评价  

Establishment and Evaluation on the Animal Model of Qi deficiency and blood stasis syndrome in hyperlipidemia rats with exhaustive swimming exercising

作　　者：张伟健 刘宏[1] 苏薇薇[1] 曾璇[1] 王永刚[1] 李沛波[1] 彭维[1] 姚宏亮[1] ZHANG Weijian;LIU Hong;SU Weiwei;ZENG Xuan;WANG Yonggang;LI Peibo;PENG Wei;YAO Hongliang(School of Life Sciences,Sun Yat-sen University Guangdong Engineering and Technology Research Center for Quality and Efficacy Re-Evaluation of Post-Marketed TCM Guangdong Key Laboratory of Plant Resources,Guangzhou 510275,China)

机构地区：[1]中山大学生命科学学院/广东省中药上市后质量与药效再评价工程技术研究中心/广东省热带亚热带植物资源与利用重点实验室,广东广州510275

出　　处：《中山大学学报（自然科学版）》2020年第2期131-136,共6页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：中山大学成果转化项目(33000-18825004);广州市民生科技攻关计划课题(201803010082)。

摘　　要：采用高脂饲喂结合力竭游泳的方法,构建新的气虚血瘀证动物模型,为相关药物研究提供有效载体。对模型大鼠进行表征观察,并检测了26个生化指标,涉及能量代谢、氧化应激、脂质代谢、免疫炎症、内皮功能以及凝血时间等多个方面。结果显示,模型大鼠呈现出精神萎靡,倦怠嗜睡,活性性差,舌质较空白对照组明显绛紫暗等反映气虚血瘀状态的典型表征,此外,与空白对照组相比,模型组上述各项生化指标均发生显著改变,提示该模型大鼠出现能量代谢紊乱,氧化应激失衡,炎症反应、免疫应答功能失调,脂质代谢异常,内皮功能紊乱,血液粘度升高等气虚血瘀发生发展相关变化。研究表明高脂饲喂结合力竭游泳能够成功建立气虚血瘀证动物模型,且该模型稳定性好,可用于心脑血管疾病药物的药效评价。The aim of the study was to establish the animal model of qi deficiency and blood stasis syndrome for drug reasearch.Rats were consecutively fed with high fat diet and then treated with exhaustive swimming exercising.The external signs of rats were observed and twenty-six biochemical parameters were detected,the parameters included energy metabolisms,oxidative stress,lipid metabolism,inflammatory cytokines,immune response,myocardial enzyme,endothelial function and clotting time etc.The results showed that the model rats were inactive,weak and appeared in lethargy,and showed a dark purple tongue,reflecting the clinical characteristics of Qi deficiency and blood stasis syndrome.Compared with the normal group,the model rats showed significant changes in the biochemical indexes mentioned above.All results together indicated that high fat diet combined with the continual exhausting swimming could cause the disorders of energy metabolisms,oxidative stress,and so on.Such disorders are closely associated with the generation and development of Qi deficiency and blood stasis syndrome.Our study demonstrated that continual exhausting swimming followed by high fat diet could be a method to make a rat model of Qi deficiency and blood stasis syndrome.The rat model could be further utilized in the evaluation of drugs for cardiovascular disease due to its good stability.

关 键 词：气虚血瘀证 动物模型 力竭游泳 高脂饲喂 

分 类 号：R965[医药卫生—药理学]