巨噬细胞抑制因子-1在慢性丙型肝炎患者外周血中的表达及意义  

Plasma macrophage inhibitory cytokine-1 levels in patients with chronic hepatitis C and its clinical significance

作　　者：叶松道[1] 叶宣梅[1] 王晓欧[1] 林巍[2] YE Songdao;YE Xuanmei;WANG Xiaoou(Center of Medical Laboratory,the Second Affiliated Hospital of Wenzhou Medical University,Wenzhou 325027,China)

机构地区：[1]温州医科大学附属第二医院检验医学学科,325027 [2]温州医科大学附属第二医院感染内科,325027

出　　处：《浙江医学》2020年第5期450-453,共4页Zhejiang Medical Journal

基　　金：浙江省医药卫生科技计划项目(2016KYB191)。

摘　　要：目的探讨慢性丙型肝炎(CHC)患者巨噬细胞抑制因子-1(MIC-1)水平的变化及其与体内病毒载量、肝脏损害程度及PR方案(聚乙二醇干扰素α+利巴韦林)抗病毒疗效的关系。方法选取CHC患者57例及同期健康体检者26例,采用双抗体夹心酶联免疫吸附法测定其血浆MIC-1水平,采用速率法、化学发光分析及实时荧光定量聚合酶链反应测定CHC患者血浆丙氨酸氨基转移酶(ALT)活性、四型胶原(C-Ⅳ)水平及丙型肝炎病毒(HCV)RNA水平。结果治疗前CHC患者血浆MIC-1水平为369.84(193.51,830.62)pg/ml,明显高于对照组的258.77(138.03,441.12)pg/ml,差异有统计学意义(P<0.05)。CHC患者中ALT升高组血浆MIC-1水平高于ALT正常组,C-Ⅳ升高组血浆MIC-1水平高于C-Ⅳ正常组,HCV RNA高水平组血浆MIC-1水平高于HCV RNA低水平组,差异均有统计学意义(均P<0.05)。CHC患者血浆MIC-1水平与ALT活性、C-Ⅳ水平及HCV RNA水平均呈正相关(r=0.322、0.423和0.333,均P<0.05)。干扰素治疗后,应答组和无应答组患者治疗前后血浆MIC-1水平比较差异均无统计学意义(均P>0.05);应答组治疗后血浆MIC-1水平低于治疗前,差异有统计学意义(P<0.05),无应答组治疗前后血浆MIC-1水平比较差异无统计学意义(P>0.05)。结论CHC患者血浆MIC-1水平升高,其水平变化在一定程度上可反映患者体内HCV病毒载量、肝脏损害程度及PR方案治疗效果,但治疗前尚不能对PR方案疗效进行预测。Objective To investigate the plasma macrophage inhibitory cytokine-1(MIC-1) levels in patients with chronic hepatitis C(CHC) and its clinical significance.Methods The levels ofplasma MIC-1 was detected by ELISA in 57 CHC patients and 26 healthy controls.ALT activity,Collagen-Ⅳ(C-Ⅳ) level and HCV RNA in plasma of CHC patients were also detected.Results The plasma MIC-1 levels in CHC patients [369.84(193.51,830.62)] were higher than those in healthy controls [258.77(138.03,441.12)](P<0.05).The MIC-1 level in patients with high HCV-RNA was significantly higher than that in patients with low HCV-RNA(P<0.05),the MIC-1 level in patients with high ALT activity was higher than that in those with low ALT activity(P<0.05);the MIC-1 level in patients with high C-Ⅳ level was higher than those with low C-Ⅳ level(P<0.05).The MIC-1 level in CHC patients was positively correlated with ALT activity,C-Ⅳ level and HCV RNA level(r=0.322,0.423,0.333,all P<0.05).There was no significant difference of the MIC-1 level between the response patients and non-response patients before and after interferon treatment(both P>0.05).The MIC-1 level in the response patients decreased significantly after treatment(P<0.05),while the MIC-1 level in the non-response patients showed no significant changes after treatment(P>0.05).Conclusion The plasma MIC-1 levels are elevated in the patients with chronic hepatitis C,and it may reflect the changes in HCV RNA load,liver damage degree and response to interferon treatment to some extent,but there is no significant predictive value for efficacy of interferon before treatment.

关 键 词：慢性丙型肝炎 丙型肝炎病毒 巨噬细胞抑制因子-1 

分 类 号：R512.63[医药卫生—内科学]