响应面试验优化霍山石斛原球茎增殖培养基  

Optimization of Protocorm Proliferation Medium of Dendrobium huoshanense by Response Surface Methodology

作　　者：郭慧慧[1] 林丛发[1] 蒋元斌[1] 李志刚 GUO Huihui;LIN Congfa;JIANG Yuanbin;LI Zhigang(Institute of Agricultural Sciences in Ningde City of Fujian Province,Fujian Ningde 355017,China)

机构地区：[1]福建省宁德市农业科学研究所,福建宁德355017

出　　处：《中国农业科技导报》2020年第3期173-180,共8页Journal of Agricultural Science and Technology

基　　金：宁德市科学技术计划项目(20160152)。

摘　　要：为可持续开发霍山石斛资源,采用响应面法优化霍山石斛原球茎增殖培养基。在单因素试验基础上,根据Box-Behnken试验设计原理,采用三因素三水平的响应面分析法,以增殖率为响应值进行回归分析。结果表明,萘乙酸(简称NAA)及6-苄氨基嘌呤(简称6-BA)浓度对增殖率影响最为显著,6-BA与马铃薯两者间交互作用显著,适宜的NAA、6-BA及马铃薯浓度分别为0.05 mg·L^-1、0.70 mg·L^-1和185 g·L^-1,此时增殖率为2989.82%±205.55%,与模型预测值基本符合。响应面法优化霍山石斛原球茎增殖培养基切实可行。In order to explore sustainable development of Dendrobium huoshanense resources,this paper used response surface method to optimize the protocorm multiplication medium.On the basis of single-factor tests,the mathematical regression model was established about the dependent variable(proliferation rate)through Box-Benhnken center composite design and response surface analysis.The results showed that the concentration of NAA and 6-benzylaminopurine(6-BA)had the most significant effect on the proliferation rate,and the interaction between 6-BA and potato was significant.The suitable concentrations of NAA,6-BA and potato were 0.05 mg·L^-1,0.70 mg·L^-1 and 185 g·L^-1,respectively.At this time,the proliferation rate was 2989.82%+205.55%,which was consistent with the predicted value of the model.Above results indicated that the response surface methodology was viable for optimizing medium composition in improving proliferation rate.

关 键 词：霍山石斛 响应面试验 原球茎增殖 培养基 优化配方 

分 类 号：S567.2[农业科学—中草药栽培]