壮医药线点灸对溃疡性结肠炎大鼠结肠组织Th17细胞及IL-17F的影响  

Effect of medicated thread moxibustion of Chinese Zhuang Minority medicine on colonic inflammatory impairment and Th17/IL-17F signaling in rats with ulcerative colitis

作　　者：张磊昌[1] 胡丽霞[2] 肖爱娇[2] 王雪[1] 裴心仪 马晓飞 葛魏 肖慧荣[1] ZHANG Lei-Chang;HU Li-Xia;XIAO Ai-Jiao;WANG Xue;PEI Xin-Yi;MA Xiao-Fei;GE Wei;XIAO Hui-Rong(Department of Anorectal,Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China;College of Traditional Chinese Medicine,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004)

机构地区：[1]江西中医药大学附属医院肛肠科,南昌330004 [2]江西中医药大学中医学院,南昌330004

出　　处：《针刺研究》2020年第3期188-193,共6页Acupuncture Research

基　　金：国家自然科学基金项目(No.81804101);江西省卫生计生委中医药科研计划项目(No.2018A364)。

摘　　要：目的:观察壮医药线点灸干预溃疡性结肠炎(UC)大鼠的疗效,并探讨其对辅助性T细胞17(Th17)/白细胞介素17F(IL-17F)轴的影响。方法:SD大鼠随机分为空白组、模型组、阳性药物组、壮医药线点灸组,每组10只。采用4%葡聚糖硫酸钠(DSS)自由饮水法制备UC大鼠模型。阳性药物组予柳氮磺胺嘧啶(SASP)灌胃,1 mL/次,2次/日,连续干预14 d;壮医药线点灸组予壮医药线点灸"天枢"和"气海"穴,1壮/穴,1次/日,双侧腧穴交替灸疗,连续干预14 d。观察记录大鼠疾病活动指数(DAI)评分;HE染色法观察大鼠结肠组织病理学变化;流式细胞术检测大鼠结肠组织中Th17细胞百分比;酶联免疫吸附法检测大鼠结肠组织中维甲酸相关孤儿核受体γt(RORγt)和IL-17F含量;荧光定量PCR法检测大鼠结肠组织RORγt和IL-17F mRNA相对表达量。结果:模型组大鼠出现粪便不成形、脓性黏液血便;镜下结肠黏膜脱落,杯状细胞消失,大量炎性细胞浸润,成纤维细胞增生;与空白组比较,模型组大鼠DAI评分、结肠组织Th17细胞百分比、结肠组织RORγt和IL-17F含量及mRNA表达量均显著升高(P<0.01,P<0.05)。与模型组比较,壮医药线点灸组和阳性药物组大鼠大便成形,无血便;结肠黏膜炎性细胞显著减少,黏膜下层水肿明显减轻;DAI评分、结肠组织Th17细胞百分比、结肠组织RORγt和IL-17F含量及mRNA表达量均显著降低(P<0.01,P<0.05)。结论:壮医药线点灸治疗UC可能与抑制结肠组织RORγt的表达,减少Th17细胞生成,降低结肠组织中促炎因子IL-17F分泌,从而减轻结肠组织的炎性损伤有关。Objective To observe the effect of medicated thread moxibustion of Zhuang Minority medicine on helper T cell 17(Th17)/Interleukin-17 F(IL-17 F) signaling pathway in ulcerative colitis(UC) rats, so as to explore its mechanisms underlying improvement of UC. Methods Forty male SD rats were randomly divided into normal control, model, medication and medicated thread moxibustion(MTM) groups, with 10 rats in each group. The UC model was induced by free drinking of 4% dextran sulfate sodium(DSS) for 10 d. After successful modeling, rats of the medication group were treated by gavage of salazosulfapyridine(SASP). Medicated thread moxibustion was applied to unilateral "Tianshu"(ST25) and "Qihai"(CV6) alternatively for rats of the MTM group, once daily for 14 d. The body mass, stool shape, and fecal occult test were recorded and conducted daily to perform disease activity index(DAI) score. H.E. staining was used to display pathological changes of the colon tissue. The Th17 cells and IL-17 F and retinoic acid related orphan receptor γ t(ROR-γt) in the colon tissue were detected by flow cytometry, and enzyme-linked immunosorbent assay(ELISA), respectively, and the expression levels of RORγt and IL-17 F mRNA in colon tissue were detected by quantitive real-time PCR. Results After modeling, the DAI score, colonic Th17 percentage, RORγt and IL-17 F contents, and RORγt and IL-17 F mRNA expression were significantly increased in the model group in contrast to the normal control group(P<0.01, P<0.05). Following the intervention, all the aforementioned indexes were reversed in both medication and MTM groups(P<0.01, P<0.05). No significant differences were found between the medication and MTM groups in the levels of the above mentioned indexes(P>0.05, except RORγt and IL-17 F mRNA expression). H.E. staining showed disappearance of goblet cells, infiltration of a large number of inflammatory cells, exfoliation of the epithelial tissue and edema of colonic mucosal in rats of the model group, which was relatively milder in both m

关 键 词：溃疡性结肠炎 壮医药线点灸 疾病活动指数 辅助性T细胞17 白细胞介素17F 

分 类 号：R245.82[医药卫生—针灸推拿学]