荧光照相法对大鼠任脉低流阻通道的活体显示  

Display of low hydraulic resistance channels along Conception Vessel by in vivo fluorography in rats

作　　者：顾鑫 王燕平[1] 王广军[2] 宋晓晶[2] 贾术永[2] 李宏彦[2] 叶丰瑶 熊枫 张维波[2] GU Xin;WANG Yan-ping;WANG Guang-jun;SONG Xiao-jing;JIA Shu-yong;LI Hong-yan;YE Feng-yao;XIONG Feng;ZHANG Wei-bo(School of Acupuncture-moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing 100029,China;Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences,Beijing 100700)

机构地区：[1]北京中医药大学针灸推拿学院,北京100029 [2]中国中医科学院针灸研究所,北京100700

出　　处：《针刺研究》2020年第3期227-232,共6页Acupuncture Research

基　　金：国家自然科学基金项目(No.81173206)。

摘　　要：目的:使用荧光照相法对大鼠任脉低流阻通道进行活体显示,为经络组织液通道学说提供影像学依据。方法:SD大鼠随机分为任脉低电阻点(LIP)组、任脉低流阻点(LHRP)组和旁开组,每组8只。将荧光素钠分别注入大鼠任脉LIP、任脉LHRP和任脉旁开点,使用荧光照相法观察荧光素钠的迁移规律。结果:任脉LIP和LHRP注射荧光素钠后均出现循经迁移轨迹,旁开组仅出现局部扩散;荧光素钠循经迁移的距离均显著长于旁开组(P<0.01),迁移线宽度均显著小于旁开组(P<0.01),循经迁移速度显著快于旁开组纵向扩散速度(P<0.01)。结论:使用荧光照相法可显示大鼠任脉循行线,为经络的组织液通道学说提供了依据,并为经络可视化研究提供了新方法。Objective To display the low hydraulic resistance channel along the running course of the Conception Vessel(CV) by using in vivo fluorography, so as to provide evidence for the existence of meridians of traditional Chinese medicine from the aspect of interstitial fluid channel. Methods A total of 24 male SD rats were randomly divided into 3 groups, namely CV-low impedance spots(CV-LIS), CV-low hydraulic resistance points(CV-LHRP) and non-meridian points(NMP) groups(n=8 in each group). The CV-LIS(the imaged line between the xiphoid process and symphysis pubis), CV-LHRP(lateral and parallel to LIS) and NMP(lateral and parallel to CV-LHRP) were detected by using meridian detector and biofluid resistance meter, respectively. Sodium fluorescein solution(1%) was injected into the spot(A-spot, upper 1/5 of the CV-LIS), and one of the NMP(about 1 cm lateral and parallel to the A-spot, and the mid-point between the Kidney and Stomach meridian running courses) by using a microsyringe for observing the migration trace of sodium fluorescein. Results After injection of fluorescein, the migration trace of fluorescein appeared along the CV-LIS and CV-LHRP lines, and only local diffusion was found in the NMP group. The migration trace lengths along the CV-LIS and CV-LHRP were significantly longer than that of the NMP group(P<0.01), and the migration width of CV-LIS and CV-LHRP groups was significantly smaller than that of the NMP group(P<0.01). The migration speed of CV-LIS and CV-LHRP groups was significantly higher than that of the NMP group(P<0.01). Conclusion Fluorography is able to be used to display the running course of CV, possibly supporting the existence of meridians from the interstitial fluid channel and providing a new method for visualization of meridians.

关 键 词：经络 低流阻通道 荧光素钠 荧光照相 任脉 

分 类 号：R245.2[医药卫生—针灸推拿学]