阿里红多糖组分对APP/PS1双转基因模型小鼠海马区AKT/GSK3β/Tau/P-tau蛋白表达的影响  

Effect of polysaccharide components from Fomes officinals Ames on expression of AKT/GSK3β/Tau/P-tau in hippocampus of APP/PS1 double transgenic model mice

作　　者：李珍 丛媛媛[1] 阿依江·哈拜克 帕丽达·阿不力孜[1] LI Zhen;CONG Yuan-yuan;AYIJIANG Ha-bai-ke;PALIDA A-bu-li-zi(College of Pharmacy,Xinjiang Medical University,Urumqi 830011,China)

机构地区：[1]新疆医科大学药学院,乌鲁木齐830011

出　　处：《天然产物研究与开发》2020年第2期288-295,共8页Natural Product Research and Development

基　　金：国家自然科学基金地区基金(81760755);新疆维吾尔自治区“十三五”重点学科(药学-高原学科)建设项目(新教研2016-7)。

摘　　要：探究阿里红多糖组分(FOPS-a和FOPS-b)对阿尔茨海默症模型小鼠海马区AKT/GSK3β/Tau/P-tau蛋白表达的影响。实验选用64只3月龄雄性APP/PS1双转基因AD模型小鼠,随机分为模型组、多奈哌齐组(0.65 mg/kg)、阿里红多糖a组分(FOPS-a)与阿里红多糖b组分(FOPS-b)高、中、低(60、30、15 mg/kg),同月龄野生型C57BL/6J小鼠为正常组,每组8只,各组分别给与药物和生理盐水灌胃给药90天。6月龄时通过跳台、旷场实验检测小鼠行为学的变化,采用尼氏染色观察各组小鼠海马区神经元形态变化,应用RT-PCR法检测小鼠海马区AKT、GSK3β、Tau mRNA转录水平,Western-blot法检测AKT、GSK3β、Tau、P-tau蛋白的表达。结果发现阿里红多糖组分(FOPS-a和FOPS-b)均能明显改善小鼠学习与记忆功能并保护海马区神经元的损伤,上调AKT mRNA转录水平和蛋白表达,下调GSK3β、Tau mRNA转录水平和GSK3β、Tau、P-tau蛋白表达。研究表明阿里红多糖组分(FOPS-a和FOPS-b)通过减少海马区神经元纤维缠结从而保护神经元的损伤,发挥拮抗AD的作用。To investigate the effects of polysaccharide components FOPS-a and FOPS-b from Fomes officinals Ames on the expression of AKT/GSK3β/Tau/P-tau in hippocampus of Alzheimer's disease model mice.The sixty-four male APP/PS1 double transgenic AD model mice were randomly divided into 8 groups as follows:model group,donepezil group(0.65 mg/kg),the high,medium and low doses of FOPS-a and FOPS-b groups(60,30,15 mg/kg)with 8 mice in each group.The same age of wild type C57BL/6J mice were included as normal group.Mice were treated with drugs and saline administered by intragastric administration for 90 days.At 6 months of age,the behavioral changes of mice were detected by jumping platform and open field test.The morphological changes of neurons in hippocampus of each group were observed by Nissl staining.RT-PCR was used to detect the transcription levels of AKT,GSK3βand Tau mRNA in hippocampus of mice.Western-blot method was used to detect AKT,GSK3β,Tau,and P-tau protein expression.The study was found that the FOPS-a and FOPS-b can significantly improve the learning and memory function of mice and protect neurons from damage in the hippocampus,up-regulates AKT mRNA transcription levels and protein expression,and down-regulates GSK3β,Tau mRNA transcription levels and GSK3β,Tau,P-tau protein expression.Studies have shown that the FOPS-a and FOPS-b can protect neurons from damage by reducing the tangles of neuron fibers in the hippocampus and play an antagonistic role in AD.

关 键 词：阿里红多糖组分 阿尔茨海默病 丝氨酸苏氨酸蛋白激酶 糖原合成酶激酶-3Β 微管相关蛋白Tau 

分 类 号：R966[医药卫生—药理学]