间接免疫荧光法与RT-PCR法检测鼠肺汉坦病毒带毒率  

Detection of hantavirusfromrat lungs by indirect immunofluorescence and RT-PCR

作　　者：杨洋[1] 张振[1] 张旭[1] 白梅[1] YANG Yang;ZHANG Zhen;ZHANG Xu;BAI Mei(Jinzhou Center for Disease Control and Prevention,Jinzhou,121001,China)

机构地区：[1]锦州市疾病预防控制中心,辽宁锦州121001

出　　处：《中华卫生杀虫药械》2020年第1期36-38,共3页Chinese Journal of Hygienic Insecticides and Equipments

基　　金：国家科技重大专项课题(编号:2107ZX10103007-001)。

摘　　要：目的对间接免疫荧光法(IFA)和逆转录聚合酶连反应(RT-PCR)两种检测鼠肺汉坦病毒(HV)的实验方法进行比较,为进一步完善出血热病毒实验室检测方案提供依据。方法采用IFA和RT-PCR对2018年收集的200份鼠肺进行HV检测、分型,统计鼠种和数量。结果锦州市鼠带汉坦病毒率2014年最高为5.42%(11/203),2010年最低为0.95%(1/105)。人间肾综合征出血热发病率2014年最高为6.77/10万,2010年最低为2.27/10万。鼠带毒指数与人间出血热发病率正相关(r=0.75,P<0.05);IFA检测鼠肺HA抗原阳性率为2.00%(4/200);RT-PCR检测鼠肺HA核酸阳性率为4.00%(8/200),两种方法检测结果有统计学差异(χ~2=13.01,P<0.01);锦州地区主要流行株为汉城(SEO)型HV。结论 RT-PCR检测鼠肺HV核酸的敏感性高于IFA检测鼠肺HV抗原的敏感性,可以推广应用RT-PCR检测鼠肺带毒率的实验室检测技术。Objective In order to provide a basis for further improving the laboratory detection scheme of hemorrhagic fever virus,the two methods of indirect immunofluorescence assay(IFA) and reverse transcription polymerase chain reaction(RT-PCR) for detection of rat pulmonary hantavirus(HV) were compared.Methods Two hundred lung samples collected in 2008 were detected and typed by IFA and RT-PCR,and the number and species of mice were counted.Results The highest HV carrying rate in rodents was 5.42%(11/203) in 2014 and the lowest was 0.95%(1/105) in 2010,while the highest human incidence of hemorrhagic fever with renal syndrome was 6.77/100 000 in 2014 and the lowest 2.27/100 000 in 2010.There was a positive correlation between the incidence of human haemorrhagic fever(r=0.75,P<0.05). The positive rate of HA antigen in rat lungs was 2.00%(4/200)when detected by IFA,and 4.00%(8/200) by RT-PCR with significant difference between the two methods(χ~2=13.01,P<0.01).The main epidemic strain in Jinzhou was Seoul(SEO) HV.Conclusion RT-PCR showed higher sensitivity than IFAin HV detection from rat lungs.It is necessary to popularize RT-PCR in the detection of HV.

关 键 词：肾综合征出血热 汉坦病毒 间接免疫荧光法 逆转录聚合酶连反应 

分 类 号：S443[农业科学—农业昆虫与害虫防治] R184.35[农业科学—植物保护]