马陆肠道细菌对普通卷甲虫活动和取食的影响  

Effect of Spirobolus bungii intestinal bacteria on the activity and feeding of Armadillidium vulgare

作　　者：陈宏敏 陈海敏 常锐锐 万晴 乐倩倩 张瑞玲 张忠 CHEN Hong-min;CHEN Hai-min;CHANG Rui-rui;WAN Qing;LE Qian-qian;ZHANG Rui-ling;ZHANG Zhong(School of Life Science of Shandong First Medical University,Tai'an 271016,China;College of Basic Medical Science of Shandong First Medical University y Tai'an 271016,China;Shandong Collaborative Innouation Center for the Origin and Control of Emerging Irifectious Diseases,Tai'an 271016,China)

机构地区：[1]山东第一医科大学生命科学学院,山东泰安271016 [2]山东第一医科大学基础医学院,山东泰安271016 [3]山东省新发传染病溯源与防控协同创新中心,山东泰安271016

出　　处：《中华卫生杀虫药械》2020年第1期56-60,共5页Chinese Journal of Hygienic Insecticides and Equipments

基　　金：国家自然科学基金项目(编号:81572028,81401693)。

摘　　要：目的探究马陆肠道细菌对普通卷甲虫取食和活动的影响,进而筛选普通卷甲虫的细菌驱避剂。方法利用传统分离纯培养法分离马陆的肠道可培养细菌,通过16S rDNA对细菌进行分子鉴定,再通过爬行痕迹实验和取食选择实验确定细菌对普通卷甲虫取食和活动的影响。结果在马陆肠道内分离到可培养肠道细菌10种,分别为短小芽孢杆菌、莫林枸橼酸杆菌、产气克雷伯氏菌、铜绿假单胞菌、肺炎克雷伯菌、蜡样芽胞杆菌、拟香味类香味菌、粪产碱杆菌、厦门芽孢杆菌、弗氏柠檬酸杆菌。通过爬行痕迹实验表明,蜡样芽胞杆菌、拟香味类香味菌和粪产碱杆菌对普通卷甲虫的活动有显著驱避作用;而通过取食选择实验表明,短小芽孢杆菌、产气克雷伯氏菌、铜绿假单胞菌、肺炎克雷伯菌、蜡样芽胞杆菌和厦门芽孢杆菌对普通卷甲虫的取食有显著驱避作用。结论明确了6种马陆肠道细菌对普通卷甲虫的取食有显著驱避作用,为开发普通卷甲虫无害化驱避剂奠定了基础。Objective To explore the effects of the bacteria from intestines of Spirobolus bungii on the feeding and activity of Armadillidium vulgare,and lay a foundation for the development of new bacterial repellents to Armadillidium vulgare.Methods The culturable bacteria from intestine of Spirobolus bungii were isolated by traditional method and identified using sequences of 16 S rDNA.The effects of bacteria on activities and feeding of A.vulgare were determined using crawling-trace experiment and feeding-selection experiment.Results A total of 10 species of culturable intestinal bacteria were isolated from the intestines of Spirobolus bungii,including Bacillus pumilus,Citrobacter murliniae,Klebsiella aerogenes,Pseudomonas aeruginosa,Klebsiella pneumoniae,Bacillus cereus,Myroides odoratimimus,Alcaligenes faecalis,Bacillus xiamenensis and Citrobacter freundii.The crawl trace experiment showed that Bacillus cereus, Myroides odoratimimus and Alkalgenes faecalis had significant repellent effect on A. vulgare activity.The feeding selection experiments showed that Bacillus pumilus,Klebsiella aerogenes,Pseudomonas aeruginosa, Klebsiella pneumoniae, Bacillus cereus and Bacillus xiamenensis had significant repellent effects on the feeding of Armadillidium vulgare.Conclusion It was clarified that the 6 kinds of intestinal bacteria from Spirobolus bungii displayed significant repellent effects on the feeding of A. vulgare,which laid foundations for the development of new harmless repellents to A. vulgare.

关 键 词：普通卷甲虫 马陆肠道细菌 爬行痕迹实验 取食选择实验 驱避剂 

分 类 号：R384.9[医药卫生—医学寄生虫学] R184.3[医药卫生—基础医学]