基于表观遗传学探索玉米双向启动子对转录的影响  

Effect of bidirectional promoter of maize on transcription based on epigenetics

作　　者：朱倩洁 曹亚欣 马宇馨 张林旺[3] 邢继红[1,2] 张康 董金皋[1,2] ZHU Qianjie;CAO Yaxin;MA Yuxin;ZHANG Linwang;XING Jihong;ZHANG Kang;DONG Jigao(College of Life Science,Hebei Agricultural University,Baoding 071000,China;Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology/Mycotoxin and Molecular Plant Pathology Laboratory of Hebei Agricultural University,Baoding 071000,China;Rongjun Hospital of Hebei Province,Baoding 071000,China)

机构地区：[1]河北农业大学生命科学学院,河北保定071000 [2]河北省植物生理与分子病理学重点实验室/河北农业大学真菌毒素与植物分子病理学实验室,河北保定071000 [3]河北省荣军医院,河北保定071000

出　　处：《河北农业大学学报》2020年第1期24-29,47,共7页Journal of Hebei Agricultural University

基　　金：国家重点研发计划-粮食丰产增效科技创新专项(2016YFD0300704);大学生创新创业训练项目(201810086066).

摘　　要：为了探索玉米中表观遗传修饰对双向启动子及相关基因表达的调控作用,本研究通过整合公共数据平台中表观基因组和转录组数据,对易接近染色质区域(Accessible chromatin regions, ACRs)和组蛋白修饰进行分析并探索其对玉米双向启动子(Bidirectional promoter, BDP)及相关基因的影响。结果发现,ACRs与转录起始位点(Transcription start site, TSS)之间的距离能够影响相应基因的表达水平:ACRs距离TSS越近,基因表达水平越高;ACRs距离TSS越远,基因表达水平越低。ACRs的分布位置在基因对的共同表达过程中起着重要的作用,这可能是通过编排BDPs周围的组蛋白修饰和开放染色质区域转录因子的结合来调节转录的。染色质结构的动态变化,ACRs和组蛋白修饰的共同作用,在调控基因组中具有BDPs的基因对及其共表达方面起着重要的作用。因此,本研究的发现可能有助于增进对ACRs和组蛋白修饰调控相关基因对转录过程的理解,为进一步探索双向启动子在玉米分子育种的功能奠定理论基础。In order to explore the regulatory role of epigenetic modification on the expression pattern of bidirectional promoters and related genes in maize,we integrated epigenome and transcriptome data in public platforms to analyze the effects of accessible chromatin regions(ACRs)and histone modifications on the transcription through the bidirectional promoter(BDP).We found that the physical location of ACRs relative to the transcription start site(TSS)can affect the expression of related genes:the closer the ACR is to the TSS,the higher the expression level of the gene.The location of ACRs plays an important role in the co-expression of gene pairs,possibly by arranging the binding of histone modifications around the BDPs and the binding of open chromatin transcription factors to regulate transcription.The dynamic interaction of chromatin structure with ACRs plays an important role in regulating the gene pairs of BDPs in the maize genome and their co-expression.Therefore,our findings may contribute to the understanding of the transcriptional processes of genes involved in ACRs and histone modification,and provide a theoretical basis for further exploration of the role of bidirectional promoters in maize molecular breeding.

关 键 词：双向启动子 易接近染色质区域 组蛋白修饰 玉米 

分 类 号：S513[农业科学—作物学]