柚子基因组比较分析以及祖先染色体重构  

Comparative analysis of pummelo genome and reconstruction of its ancestor

作　　者：袁嘉庆 王金朋 王希胤 YUAN Jiaqing;WANG Jinpeng;WANG Xiyin(School of Life Sciences,North China University of Science and Technology,Tangshan 063210,China)

机构地区：[1]华北理工大学生命科学学院,河北唐山063210

出　　处：《河北农业大学学报》2020年第1期48-54,61,共8页Journal of Hebei Agricultural University

基　　金：国家自然科学基金(3117022);华北理工大学研究生创新项目(2018S42).

摘　　要：柚子(Citrus grandis Osbeck)全基因组测序完成为柑橘植物乃至整个芸香科植物提供了研究机会。本研究通过对柚子基因组结构分析,鉴定柚子基因组中存在的由多倍化产生的重复基因,构建了葡萄基因组和柚子基因组之间与多倍化、物种分化相关联的同源共线性基因列表,为柚子基因组进化和基因功能演化提供了重要的比较基因组学数据资源;通过与外类群葡萄保留的同源染色体区域进行比较,发现柚子基因组在多倍化之后发生了大量的片段化重组;利用葡萄基因组保留有较完整的染色体结构相对于真双子叶六倍体(ECH),结合葡萄和柚子基因组之间的同源关系,推断出柚子祖先基因组结构。本研究对理解柚子基因组乃至柑橘植物基因组以及其进化提供了重要的比较基因组学的基础。With rich nutrition in fruits,pummelo has very important economic value.The sequencing of genome structure of pummelo has been completed,which provides an opportunity to study Citrus plants and the Rutaceae plants.By comparatively analyzing the relationships among genomes and within genomes,we deduced the duplicated genes produced by polyploidization and indentified the orthologies between the compared genomes.After then,we performed a colinear list between grape and pummelo associated with polyploidization and speciation,which provided an important genomic comparative resource to study the genome evolution and gene functions of pummelo.Comparing with the homologous region of the outgroup grape,we found that the pummelo had suffered severe fragmentation recombination.Furthermore,considering the outgrowp grape genome with a relatively complete structure as to ECH,we deduced the ancestor genome structure of pummelo.Finally,this study provides an important comparative genomics resource for understanding the evolution of pummelo,even of Citrus plants.

关 键 词：柚子 共线性 多倍化 片段化 染色体重构 

分 类 号：Q949.752.7[生物学—植物学]