紫花苜蓿4个生理指标在冬季自然条件下的变化规律研究  

Study on the changes of 4 physiological indexes of alfalfa in winter under natural conditions

作　　者：尹卫 杨国柱 李小安 马蓉蓉 熊丽丽 朱星伟 祁小清 柴晓燕 YIN Wei;YANG Guozhu;LI Xiaoan;MA Rongrong;XIONG Lili;ZHU Xingwei;QI Xiaoqing;CAI Xiaoyan(State Key Laboratory of Plateau Ecology and Agriculture,Qinhai University,Xining 810016,China;College of Eco-Environmental Engineering,Qinghai University,Xining 810016,China;Qinghai JinyunEnvironmentalTechnology Co.,LTD.,Xining 810000,China;Gansu Agricultural University,Lanzhou 730070,China;Baiyin industry and Information Committee,Baiyin 730900,China)

机构地区：[1]省部共建三江源生态与高原农牧业国家重点实验室,西宁810016 [2]青海大学生态环境工程学院,西宁810016 [3]青海金云环境科技有限公司,西宁810000 [4]甘肃农业大学,兰州730070 [5]白银市工业和信息化委员会,甘肃白银730900

出　　处：《黑龙江畜牧兽医》2020年第3期93-96,99,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家重点研发计划项目“基于大数据的高寒草地退化沙化评价方法与模型构建”(2016YFC0501801);青海省科技厅科技成果转化专项“青海农区苜蓿草+玉米青贮发展牛羊生态养殖循环产业链示范”(2016-NK-140);青海省科技厅科技计划项目“基于大数据挖掘和高寒草地退化评价”(2017-ZJ-730);青海省科技厅自然科学基金项目“G6PDH和AOX在紫花苜蓿冷适应性中的响应机制研究”(2016-ZJ-930Q);青海省科技厅应用基础研究计划项目“青大1号紫花苜蓿在环湖地区高寒气候条件下的生理适应机制研究”(2015-ZJ-713);海南州科技局科技合作计划项目“环湖南岸地区豆科牧草修复天然草地研究”;海南州科技局科技合作计划项目“青大系列紫花苜蓿在环湖南岸适应性研究”;青海大学中青年基金项目“青大系列紫花苜蓿对比研究”。

摘　　要：为了研究紫花苜蓿在青藏高原高寒气候条件下安全越冬的机制,试验于2016年11月1日—2017年3月1日开展了青大1号紫花苜蓿冬季4个生理指标变化规律的研究,试验期间每隔10 d采集1次青大1号紫花苜蓿的越冬芽样品,测定越冬芽中可溶性蛋白、可溶性糖、脯氨酸含量及相对电导率,对相关数据进行单因素方差分析,同时对数据开展隶属函数和灰色关联度分析。结果表明:不同月份间青大1号紫花苜蓿越冬芽中可溶性蛋白、可溶性糖、脯氨酸含量以及相对电导率值存在显著差异(P<0.05);4个生理指标与抗寒性关联程度顺序为脯氨酸含量>可溶性糖含量=相对电导率>可溶性蛋白含量。说明脯氨酸的含量与青大1号紫花苜蓿抗寒性的关系最密切,且各项抗寒指标的关联度都在0.6以上。In order to study the mechanism of alfalfa overwintering safely under the high cold climate in Qinghai-Tibet Plateau,the experiment was carried out from November 1,2016 to March 1,2017 to study the change regularity of four physiological indexes of Alfalfa Qingda No.1 in winter.Duringthe experiment,the overwintering buds of Qingda No.1 Alfalfa were collected once every 10 days,and were used to determine the content of soluble protein,soluble sugar,and proline and the relative conductivity.The related data were analyzed by one-way ANOVA,mem bership function and grey correlation analysis.The results showed that the contents of soluble protein,soluble sugar,and proline and the relative conductivity of overwintering bud of Alfalfa Qingda No.1 were significantly different in different months(P<0.05);the correlation degree of four physiological indexes with cold resistancewas proline content>soluble sugar content=relative conductivity>soluble protein content.The results indicated that the content of proline was most closely related to the cold resistance of Qingda No.1 alfalfa,and the correlation degree of each cold resistance index was above 0.6.

关 键 词：冬季 紫花苜蓿 越冬芽 生理指标 抗寒性 隶属函数 灰色关联度分析 

分 类 号：S311[农业科学—作物栽培与耕作技术] S541[农业科学—农艺学]