消痛灵喷雾剂的工艺优化及对小鼠的抗炎镇痛作用  

Study on Process Optimization and Anti-Inflammatory and Analgesic Effect of Xiaotongling Spray

作　　者：梁语嫣 彭梅[2] 杨小生 陈发菊 罗忠圣 杨礼寿 邓廷飞 王丽[2] 王道平[2] 杨娟 LIANG Yuyan;PENG Mei;YANG Xiaosheng;CHEN Faju;LUO Zhongsheng;YANG Lishou;DENG Tingfei;WANG Li;WANG Daoping;YANG Juan(School of Pharmacology,Guizhou Medical University,Guiyang 550025,Guizhou,China;Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences,Guiyang 550014,Guizhou,China)

机构地区：[1]贵州医科大学药学院,贵州贵阳550025 [2]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550014

出　　处：《贵州医科大学学报》2020年第2期174-178,共5页Journal of Guizhou Medical University

基　　金：贵州省科技计划项目[黔科合(2016)支撑2824];贵阳市高新区科技项目(GXCX-2018-001)。

摘　　要：目的:对消痛灵喷雾剂的制备工艺优化,观察最优工艺制备的喷雾剂对小鼠的抗炎镇痛作用。方法:采用L9(43)正交实验,以水杨酸含量为考察指标,优选出最佳制备工艺;将小鼠随机分为空白组、阳性组和消痛灵高、中、低剂量组,采用二甲苯致小鼠耳肿胀法、蛋清致小鼠足趾肿胀法观察最佳工艺制备的喷雾剂对小鼠的抗炎作用,采用醋酸扭体法观察喷雾剂对小鼠的镇痛作用。结果:影响消痛灵喷雾剂中水杨酸提取率的各因素主次顺序为浸泡天数、乙醇浓度、料液比及粉粒度,最佳制备工艺的浸泡时间为7 d,乙醇浓度为75%,液料比为1∶5,药材过10目筛;最佳工艺制备的喷雾剂能显著降低二甲苯所致试验小鼠耳廓肿胀(P<0.05)及蛋清引起的小鼠足跖肿胀(P<0.05),能够减少醋酸所致小鼠扭体次数(P<0.05),且与阳性药效果相当。结论:优选出消痛灵喷雾剂最佳制备工艺,最佳工艺条件下制备的喷雾剂具有较好的抗炎镇痛作用。Objective:To optimize process and study the anti-inflammatory and analgesic effects of xiaotongling spray.Methods:L9(43)orthogonal experiment was adapted to optimize the preparation process with salicylic acid content as the index.The mice were randomly divided into blank group,positive control group and groups with high,medium and low doses of xiaotongling spray.Xylene-induced auricle swelling and toe swelling induced by egg white method were applied to observe the anti-inflammatory effects of xiaotongling spray,which was produce with prime process;the acetic acid writhing method in mice was taken to observe the analgesic effects of xiaotongling spray.Results:Influencing order of salicylic acid extraction rate in xiaotongling spray was as follows:immersion time(A)>ethanol dose(B)feed liquid rate(C)particle size(D);optimum process as follows:immersing for 7 days,the ethanol dose as 80%,feed liquid rate as 1∶5 and the powder particle size was 10 mesh sieve.Xiaotongling spray can significantly inhibit xylene-induced auricle swelling(P<0.05)and egg white-induced foot swelling in mice(P<0.05).Xiaotongling spray can also reduce the writhing times induced by glacial acetic acid in mice(P<0.05),and it equal to the positive drug effect.Conclusion:The optimum process and the Xiaotongling spray produced in such process has obvious anti-inflammatory and analgesic effects.

关 键 词：中草药 工艺学 制药 消炎药 非甾类 水杨酸 消痛灵喷雾剂 

分 类 号：R28[医药卫生—中药学]