基于Android平台便携式荧光免疫层析试条检测装置的设计  

Design of a portable fluorescent immunochromatographic strip detection device based on Android platform

作　　者：郑齐 吴辉煌[1,3] 高跃明 Zheng Qi;Wu Huihuang;Gao Yueming(College of Physics and Telecommunication Engineering,Fuzhou University,Fuzhou 350108;Fuzhou University Zhicheng College,Fuzhou 350002;Key Lab of Medical Instrumentation and Pharmaceutical Technology of Fujian Province,Fuzhou 350108)

机构地区：[1]福州大学物理与信息工程学院,福州350108 [2]福州大学至诚学院,福州350002 [3]福州大学福建省医疗器械和医药技术重点实验室,福州350108

出　　处：《电气技术》2020年第4期10-14,共5页Electrical Engineering

基　　金：福建省科技重大专项(2014YZ0001).

摘　　要：荧光免疫层析定量分析具有精度高、检测速度快、操作简单等优点,广泛应用于环境保护、食品安全、临床医疗等领域。本文基于Android平台、OpenCV计算机视觉库的图像降噪等处理技术,开发便捷操作的人机交互应用程序;制作便于携带且与手机匹配良好的荧光试条图像采集暗盒;设计滑动窗口图像分割算法,提取试条测试线、质控线和背景区域的灰度值,最终计算出试条的特征值。使用标准试条图片,对装置检验结果的重复性、有效性、计算耗时等进行测试。实验结果表明:检测装置准确定位荧光试条图像中的测试线和质控线;在试条有效测试范围内,测试线的灰度值降低时,荧光试条图像特征值随之单调递减;改变滑动窗口的宽度对特征值的影响微小。Quantitative analysis of fluorescence immunochromatographic assay(FICA)has many advantages,such as high accuracy,fast detection speed and simple operation.It has been widely used in environmental protection,food safety,clinical medicine and others.Based on Android platform and image denoising technology of OpenCV computer vision library,a man-computer interactive application with convenient operation is developed;a portable fluorescent strip image acquisition dark box that matches well with the mobile phones is made;a sliding window image segmentation algorithm is designed to extract the gray values of the test line,control line and background area,and finally calculate the characteristic value of the test strip.The repeatability,validity and calculation time of the test results are tested by using standard strip pictures.The experimental results show that the detection device locate the test line and the control line of fluorescent strip images accurately;the characteristic values of fluorescent strip images decrease monotonously with the decrease of the gray values of the test line within the effective test range;changing the width of the sliding window has little effect on the characteristic value.

关 键 词：荧光免疫层析 ANDROID系统 定量检测 便携 滑动窗口 

分 类 号：TH77[机械工程—仪器科学与技术] TP311.56[机械工程—精密仪器及机械] TP391.41[自动化与计算机技术—计算机软件与理论]