夹脊电针联合甲基强的松龙对急性脊髓损伤模型大鼠NMDA-NR1、Calpain-2和cleaved caspase-3表达影响的实验研究  

An Experimental Study on the Effects of Spinal Acupuncture Combined with Methylprednisolone on The Expressions of NMDA-NR1,Calpain-2 and Cleaved Caspase-3 in Rats with Acute Spinal Cord Injury

作　　者：付豪[1] 李诺[1] 李晓宁[2] FU Hao;LI Nuo;LI Xiaoning(Heilongjiang University of Chinese Medicine,Harbin 150040, China;The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150001, China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]黑龙江中医药大学附属第二医院,黑龙江哈尔滨150001

出　　处：《针灸临床杂志》2020年第3期55-60,共6页Journal of Clinical Acupuncture and Moxibustion

基　　金：国家自然科学基金面上项目,编号:81373715;哈尔滨市应用技术研究与开发项目科技创新人才类(优秀学科带头人B类),编号:2016RAXYJ090。

摘　　要：目的:观察夹脊电针联合甲基强的松龙对急性脊髓损伤大鼠脊髓组织NMDA-NR1、Calpain-2和cleaved caspase-3蛋白表达的影响。方法:120只雌性Wistar大鼠被均分为下述几组,即正常组(Normal组)、急性脊髓损伤+抑制剂组(MDL组)、急性脊髓损伤+甲基强的松龙组+夹脊电针治疗(EA+MP组)、急性脊髓损伤组(ASCI组)与假手术组(Sham组),各24只。对于这些研究组,又将其分为4个不同的亚组,即1天、3天、7天、14天亚组,每组大鼠均为6只。假手术组仅暴露脊髓,手术后常规饲养;其余各组于模型成功后分别进行相应处置,选择BBB评分为1~3分的大鼠入组。大鼠于术后第14天,在治疗时间点结束后再以BBB法测定其运动功能,然后处死并取材,以Western blot方法检测其NMDA-NR1、Calpain-2和cleaved caspase-3蛋白的表达。实验结果使用SPSS19.0统计软件进行统计学分析。结果:BBB评分结果显示,在所研究的模型组中,BBB评分明显地低于假手术组(P<0.05);在对大鼠进行相关的治疗之后,EA+MP组14天后的BBB评分显著升高(P<0.05)。EA+MP组和MDL组大鼠的NMDA-NR1蛋白表达均明显低于ASCI组(P<0.05),且EA+MP组NMDA-NR1蛋白表达低于MDL组(P<0.05)。MDL组以及EA+MP组大鼠Calpain-2蛋白表达明显低于ASCI组(P<0.05)。EA+MP组大鼠cleaved caspase-3蛋白表达明显低于MDL组(P<0.05)。结论:夹脊电针联合甲基强的松龙能降低急性脊髓损伤模型大鼠NMDA-NR1、Calpain-2和cleaved caspase-3蛋白表达,并提高生活质量。Objective:To observe the effect of Jiaji electro-acupuncture combined with Methylprednisolone(MP)on the protein expressions of NMDA-NR1,Calpain-2 and cleaved caspase-3 in spinal cord tissue of rats with acute spinal cord injury(ASCI).Methods:120 female Wister rats were randomly divided into the normal group,the sham operation group,ASCI group,EA+MP group and MDL28170 group,with 24 rats in each group.Each group was subdivided into subgroups of 1 d,3 d,7 d and 14 d,with 6 rats in each subgroup.The rats in the sham operation group were only intervened to expose spine cord,and then were raised normally after operation.Rats with BBB score of 1-3 were selected into the other groups after modeling.After 14days of corresponding treatment,the injured spinal cord was removed and the protein expressions of NMDA-NR1,Calpain-2 and cleaved caspase-3 were detected by using Western blot method.The experimental data were analyzed by SPSS19.0 software.Results:The BBB scores were significantly lower in the model groups than those in the sham operation group(P<0.05);the BBB scores were increased more significantly in EA+MP group after 14 days of treatment(P<0.05).Western blot test showed that the protein expressions of NMDA-NR1,Calpain-2 and cleaved caspase-3 were significantly lower in EA+MP group and MDL28170 group than those in ASCI group(P<0.05);the expressions were lower in EA+MP group than those in the MDL28170 group(P<0.05).Conclusion:Jiaji electro-acupuncture combined with Methylprednisolone can decrease the protein expressions of NMDA-NR1,Calpain-2 and cleaved caspase-3 in rats with ASCI.

关 键 词：夹脊 电针 甲基强的松龙 脊髓损伤 微环境 细胞凋亡 

分 类 号：R246.2[医药卫生—针灸推拿学]