地塞米松联合重组BPI蛋白干预对肺炎支原体感染小鼠的肺功能及AEC-Ⅱ细胞超微结构和血清sB7-H3、sPD-1水平的影响  

Effects of Dexamethasone Combined with Recombinant BPI Protein on Pulmonary Function,AEC-II Cell Ultrastructure and Serum sB7-H3 and sPD-1 Levels in Mice Infected with Mycoplasma Pneumoniae

作　　者：王晶晶 袁文杏 张向民[1] 郭陶陶 刘敏 WANG Jingjing;YUAN Wenxing;ZHANG Xiangmin;GUO Taotao;LIU Min(Department of the Second Hospital of Handan, Handan 056002, China;The Central Hospital of Handan,Handan 056000, China)

机构地区：[1]邯郸市第二医院呼吸内科,河北邯郸056002 [2]邯郸市中心医院呼吸内科,河北邯郸056000

出　　处：《标记免疫分析与临床》2020年第3期508-512,共5页Labeled Immunoassays and Clinical Medicine

基　　金：河北省医学科学研究课题计划(编号:20191792)。

摘　　要：目的探究并分析地塞米松联合重组杀菌性/通透性增加蛋白(bactericidal/permeability-enhancing protein,BPI)对肺炎支原体感染小鼠肺功能、肺泡Ⅱ型上皮细胞(alveolar epithelial cell typeⅡ,AEC-Ⅱ)超微结构及可溶性B7-H3(sB7-H3)及可溶程序性死亡分子-1(soluble programmed death molecule-1,sPD-1)表达的影响。方法选取健康小鼠120只(清洁级,体重20~25g),按照随机数字表法将其分为对照组和观察组,每组60只。所有小鼠均肺炎支原体感染造模成功(经过肺炎支原体感染)。对照组小鼠又随机分为未处理组、处理后1周组(地塞米松处理)、处理后2周组(地塞米松处理),每组20只。观察组小鼠又随机分为未处理组、处理后1周组(地塞米松+重组BPI蛋白)、处理后2周组(地塞米松+重组BPI蛋白),每组20只。观察两组小鼠处理前、处理后1周、处理后2周肺功能、AEC-Ⅱ细胞超微结构、sB7-H3、sPD-1相对表达量。结果两组小鼠处理前后肺功能差异具有统计学意义(P<0.05),处理后两组小鼠肺功能显著提高,且观察组小鼠处理1周、2周肺功能显著高于对照组(P<0.05);处理前两组小鼠AEC-II超微结构差异无统计学意义,处理后观察组小鼠AEC-II超微结构显著比对照组小鼠正常。经重复方差分析,两组小鼠处理前后sB7-H3、sPD-1水平差异具有统计学意义(P<0.05),处理后,两组小鼠sB7-H3、sPD-1水平显著降低(P<0.05),处理1周、2周观察组小鼠sB7-H3、sPD-1水平明显低于对照组(P<0.05),差异具有统计学意义。结论重组BPI蛋白联合地塞米松能有效降低肺炎支原体感染小鼠sB7-H3及sPD-1表达,增强肺炎支原体感染小鼠肺功能,减少AEC-Ⅱ细胞超微结构损伤。Objective To investigate and analyze the effects of dexamethasone combined with recombinant BPI protein on pulmonary function,ultrastructure of AEC-II cells and expression of soluble B7-H3(sB7-H3)and soluble programmed death molecule-1(sPD-1)in mice infected with Mycoplasma pneumoniae.Methods 120 healthy mice(clean grade,weight 20-25 g)were divided into the control group and the observation group according to random number table method,with 60 mice in each group.All mice were successfully infected with Mycoplasma pneumoniae(by infection with Mycoplasma pneumoniae).The mice in the control group were randomly divided into the untreated group,one-week group(treated with dexamethasone)and two-weeks group(treated with dexamethasone),with 20 mice in each group.The mice in the observation group were randomly divided into the untreated group,one-week after treatment group(dexamethasone+recombinant BPI protein),and two-weeks after treatment group(dexamethasone+recombinant BPI protein),with 20 mice in each group.The lung function,ultrastructure of AEC-II cells,relative expression of sB7-H3 and sPD-1 were observed before treatment,1 week and 2 weeks after treatment.Results There was significant difference in lung function between the two groups before and after treatment(P<0.05).After treatment,the lung function of the two groups increased significantly,but the lung function of the observation group was significantly higher than that of the control group(P<0.05)after treatment for one week and two weeks;also there was no significant difference in the ultrastructure of AEC-II between the two groups before treatment,and the ultrastructure of aec-ii in the observation group was significantly better than that of the control group.After treatment,the levels of sb7-h3 and sPD-1 in the two groups were significantly lower than those in the control group(P<0.05).Conclusion Recombinant BPI protein combined with dexamethasone can effectively reduce the expressions of sB7-H3 and sPD-1 in mice infected with Mycoplasma pneumoniae,further

关 键 词：肺炎支原体感染 小鼠 地塞米松 AEC-Ⅱ细胞超微结构 

分 类 号：R375.2[医药卫生—病原生物学]