脑心肌炎病毒感染Hela细胞的内吞途径  

Endocytic Pathway of EMCV Infected Hela Cells

作　　者：刘杨 许淑娟 李琼毅[1,2] 刘翊忠 LIU Yang;XU Shujuan;LI Qiongyi;LIU Yizhong(Biomedical Research Center, Northwest Minzu University, Lanzhou 730030, China;Life Science and Engineering College, Northwest Minzu University, Lanzhou 730030, China)

机构地区：[1]西北民族大学生物医学研究中心,兰州730030 [2]西北民族大学生命科学与工程学院,兰州730030

出　　处：《西北农业学报》2020年第2期189-197,共9页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：西北民族大学引进人才科研启动项目(xbmuyjrc201627);甘肃省教育厅项目(2018B-018);中央高校基本科研业务项目(31920180123)。

摘　　要：旨在探究脑心肌炎病毒(Encephalomyocarditis virus,EMCV)是否通过内吞途径以及通过何种内吞途径感染细胞,分别采用内吞途径抑制剂、网格蛋白抑制剂、巨胞饮途径抑制剂和肌动蛋白抑制剂作用于Hela细胞后接种EMCV,通过TCID50、qRT-PCR和Western blot等方法检测病毒感染是否受影响。结果显示,内吞途径抑制剂和肌动蛋白抑制剂作用于Hela细胞后,TCID50、qRT-PCR和Western blot等检测结果提示EMCV的感染受到抑制。表明EMCV可通过肌动蛋白参与的内吞途径感染Hela细胞。To investigate whether encephalomyocarditis virus(EMCV)infected Hela cells depends on endocytic pathway and depends on which kind of endocytic pathway,the Hela cells were treated with specific inhibitors of endocytic pathway,clathrin,macropinocytosis and actinseparately,and infected by EMCV,then the change of virus infection was detected through TCID50,qRT-PCR and Western blot.The detected results of TCID50,qRT-PCR and Western blot showed that specific inhibitors of endocytic pathway and actin could inhibit EMCV infection of Hela cells.Finally,it was confirmed that EMCV could infects Hela cells through the endocytic pathway involving actin.

关 键 词：肌动蛋白 脑心肌炎病毒 人宫颈癌细胞 

分 类 号：Q95[生物学—动物学]