不同运动强度干预2型糖尿病模型大鼠的内脂素及糖代谢变化  

Effects of different exercise intensities on visfatin level and glucose metabolism in type 2 diabetic rats

作　　者：李颖 林文弢[2] 翁锡全[2] Li Ying;Lin Wentao;Weng Xiquan(Zhaoqing Physical Education School,Zhaoqing 526000,Guangdong Province,China;Guangzhou Sport University,Guangzhou 510500,Guangdong Province,China)

机构地区：[1]肇庆市体育学校,广东省肇庆市526000 [2]广州体育学院,广东省广州市510500

出　　处：《中国组织工程研究》2020年第26期4196-4200,共5页Chinese Journal of Tissue Engineering Research

基　　金：广东省教育厅自然科学重点项目(2013KJCX0115),项目负责人:翁锡全。

摘　　要：背景:内脂素具有胰岛素样的降糖作用,在2型糖尿病的治疗中可能有着积极的意义,不同运动强度对其的影响也不同。目的:观察6周不同强度有氧运动干预2型糖尿病大鼠糖代谢的效应及内脂素变化的特点并探讨其关系。方法:采用高脂饲料喂养并注射链脲佐菌素的方法,建立雄性SD大鼠2型糖尿病模型,将造模成功的43只大鼠随机分为糖尿病安静对照组10只,糖尿病运动Ⅰ,Ⅱ,Ⅲ组各11只。此3个运动组大鼠运动强度分别为10,15,20m/min,运动量均为1h/d,每周5d,持续运动6周后采集血样,检测糖代谢相关指标、血清及内脏脂肪内脂素水平。结果与结论:(1)实验中3个运动组糖代谢相关指标(空腹血糖、糖化血清蛋白、胰岛素及胰岛素抵抗指数等)均出现了改善,同时糖尿病运动Ⅰ,Ⅱ组血清内脂素水平均出现非常显著性降低(P<0.01),而糖尿病运动Ⅲ组虽有一定程度下降,但无显著性意义(P>0.05);(2)糖尿病运动Ⅰ,Ⅱ,Ⅲ组内脏脂肪内脂素水平出现了显著性下降(P<0.05),其中糖尿病运动Ⅱ内脏脂肪内脂素下降最显著(P<0.01);(3)结果证实,中、低强度有氧运动能更有效改善2型糖尿病大鼠胰岛素抵抗状态,降低血糖浓度,且与内脏脂肪减少和内脂素分泌减少有关,内脂素可能起了积极的作用。BACKGROUND:Visfatin has an insulin-like hypoglycemic effect with positive significance in the treatment of type 2 diabetes.Different exercise intensities have different effects on the level of visfatin.OBJECTIVE:To observe the effects of 6-week aerobic exercise of different intensities on glucose metabolism and the level of visfatin in type 2 diabetic rats and to explore the relationship between them.METHODS:The male Sprague-Dawley rats were fed with high-fat diet and injected with streptozotocin to establish a type 2 diabetes model.The 43 model rats were randomly divided into quiet control group(n=10),exercise group I(n=11),exercise group II(n=11)and exercise group III(n=11).The exercise intensities of the three exercise groups were 10,15,and 20 m/min,respectively.Exercises of different intensities were performed 1 h/d,5 days per week,for 6 continuous weeks.Blood samples were collected at the end of 6 weeks for determination of glucose metabolism-related indicators and serum and visceral levels of visfatin.RESULTS AND CONCLUSION:The indexes related to glucose metabolism(fasting blood glucose,glycosylated serum protein,insulin,insulin resistance index,etc.)in the three exercise groups were improved.The serum level of visfatin in the exercise groups I and II were significantly decreased(P<0.01),while the serum level of visfatin in the exercise group III was decreased to some extent,but not significantly(P>0.05).There was a significant decrease in visfatin level in the three exercise groups(P<0.05),especially in the exercise group II(P<0.01).The results indicate that low-intensity aerobic exercise can effectively improve insulin resistance and reduce blood glucose concentration in type 2 diabetic rats,and it is related to the loss of visceral fat and the reduction of visfatin secretion,where visfatin may play a positive role.

关 键 词：有氧运动 大鼠 2型糖尿病 内脂素 内脏脂肪 胰岛素抵抗 高脂饮食 胰岛素 糖代谢 链脲佐菌素 

分 类 号：R454[医药卫生—治疗学] R363[医药卫生—临床医学]