三七皂苷R1保护四氯化碳诱导肝纤维化模型大鼠的作用  

Protective effect of notoginsenoside R1 on carbon tetrachloride-induced liver fibrosis in rats

作　　者：吴朕 马微[2] 臧成昊 刘矿嫔 刘伟[2] 刘洁 梁宇 李春艳[2] 陈志明 茹金 樊楚明 杨金伟 郭建辉 李力燕[2] Wu Zhen;Ma Wei;Zang Chenghao;Liu Kuangpin;Liu Wei;Liu Jie;Liang Yu;Li Chunyan;Chen Zhiming;Ru Jin;Fan Chuming;Yang Jinwei;Guo Jianhui;Li Liyan(The Affiliated Hospital of Kunming University of Science and Technology,the First People’s Hospital of Yunnan Province,Kunming 650032,Yunnan Province,China;Kunming Medical University,Kunming 650500,Yunnan Province,China)

机构地区：[1]昆明理工大学附属医院,云南省第一人民医院,云南省昆明市650032 [2]昆明医科大学,云南省昆明市650500

出　　处：《中国组织工程研究》2020年第26期4213-4217,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(31560295),项目负责人:李力燕;云南省科技厅昆明医科大学联合专项重点项目[2018FE001(-163)],项目负责人:李力燕;云南省科技厅昆明医科大学联合专项重点项目[2019FE001(-179)],项目负责人:樊楚明;云南省心血管疾病影像研究中心(2017NS254,2018NS0270),项目负责人:陈志明;云南省创新团队(2019HC022),项目负责人:李力燕;云南省万人计划(YNWR-MY-2018-015),项目负责人:郭建辉;昆明医科大学重大科技成果培育项目(CGPY201802),项目负责人:李力燕。

摘　　要：背景:前期研究发现,三七总皂苷对小鼠免疫性肝损伤具有一定的保护作用。目的:探究三七皂苷R1对四氯化碳诱导的肝纤维化模型大鼠的治疗作用。方法:用四氯化碳诱导SD雄性大鼠制备肝纤维化模型,给药组按照60 mg/kg的剂量给予30 g/L三七皂苷R1溶液,1次/d,连续4周和6周。对照组及模型组给予同体积的生理盐水,采用苏木精-伊红染色及Masson染色观察肝脏组织结构和纤维化程度分期;反转录-定量聚合酶链反应(q RT-PCR)检测法检测Ⅰ型胶原、α-平滑肌激动蛋白和转化生长因子β1表达水平。实验方案经昆明医科大学动物实验伦理委员会批准(批准号为approval No.KMMU2018018)。结果与结论:(1)肝组织病理学显示,与模型组相比,三七皂苷R1能显著减轻纤维增生程度;(2)与模型组相比,三七皂苷R1组Ⅰ型胶原、α-平滑肌激动蛋白和转化生长因子β1表达水平显著降低(P<0.05),三七皂苷R1给药4周与6周组比较差异无显著性意义;(3)结果提示,三七皂苷R1对四氯化碳诱导的肝纤维化模型大鼠具有一定的治疗作用。BACKGROUND:Previous studies have found that panax notoginseng saponins have a certain protective effect on immunological liver injury in mice.OBJECTIVE:To explore the therapeutic effect of notoginsenoside R1 on carbon tetrachloride-induced liver fibrosis in rats.METHODS:Experimental liver fibrosis model was made by carbon tetrachloride in male Sprague-Dawley rats.Then 30 g/L notoginsenoside R1(60 mg/kg)was given once daily for 4 and 6 weeks in the treatment group.Rats in the control and model group were given distilled water of the same volume.Histopathological observation with hematoxylin-eosin staining and Masson’s trichrome staining was used to evaluate the changes of liver structure and fibrosis degree.The expression of collage type I,α-smooth muscle actin and transforming growth factor-β1 mRNA of hepatic tissue was measured by qRT-PCR method.The experimental protocol was approved by the Animal Experiment Ethics Committee of Kunming Medical University(approval No.KMMU2018018).RESULTS AND CONCLUSION:Liver histopathology showed that notoginsenoside R1 improved the degree of liver fibrosis.The expression levels of collagen type I,α-smooth muscle actin and transforming growth factor-β1 mRNA were reduced significantly in the treatment group compared with the model group(P<0.05).But there was no significant difference after 4 and 6 weeks of treatment with notoginsenoside R1.Overall findings indicate that notoginsenoside R1 can slow down the progression of carbon tetrachloride-induced liver fibrosis in rats to a certain extent.

关 键 词：三七皂苷R1 四氯化碳 肝纤维化 Ⅰ型胶原(Col-1) Α-SMA 转化生长因子Β1 

分 类 号：R446[医药卫生—诊断学] R496[医药卫生—临床医学]