α2受体在右美托咪定改善创伤性颅脑损伤大鼠肠道屏障功能障碍中的作用  

Role of α2 receptor in dexmedetomidine-induced improvement in intestinal barrier dysfunction in rats with traumatic brain injury

作　　者：张红涛[1] 王焕宇[2] 刘玲玲[1] 王红光[2] 于泳浩[3] 陈君[1] Zhang Hongtao;Wang Huanyu;Liu Lingling;Wang Hongguang;Yu Yonghao;Chen Jun(Department of Anesthesiology,Tianjin Huanhu Hospital,Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases,Tianjin 300350,China;Department of Neurosurgery,Tianjin Huanhu Hospital,Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases,Tianjin 300350,China;Department of Anesthesiology,Tianjin Medical University General Hospital,Tianjin 300052,China)

机构地区：[1]天津市环湖医院麻醉科,天津市脑血管病与神经变性重点实验室,300350 [2]天津市环湖医院神经外科,天津市脑血管病与神经变性重点实验室,300350 [3]天津医科大学总医院麻醉科,300052

出　　处：《中华麻醉学杂志》2019年第11期1391-1394,共4页Chinese Journal of Anesthesiology

摘　　要：目的评价α2受体在右美托咪定改善创伤性颅脑损伤(TBI)大鼠肠道屏障功能障碍中的作用。方法清洁级健康雄性Wistar大鼠40只,12周龄,体重200~250 g,采用随机数字表法分为4组(n=10):对照组(C组)、TBI组(T组)、右美托咪定组(D组)和右美托咪定+阿替美唑组(D+A组)。采用自由落体撞击法建立大鼠TBI模型,于模型制备前30 min饲入4.0 kD异硫氰酸荧光素标记的右旋糖酐(FITC-Dex)60 mg/100 g;D组和D+A组于模型制备前30 min腹腔注射右美托咪定50μg/kg,D+A组于注射右美托咪定前10 min腹腔注射阿替美唑500μg/kg。于模型制备后6 h时采集颈总动脉血样,采用ELISA法检测血浆肾上腺素(E)和去甲肾上腺素(NE)的浓度,采用荧光分光光度计法测定血清FITC-Dex浓度,采用比色法测定血清二胺氧化酶(DAO)活性;取小肠组织,采用比色法测定肠组织DAO活性,检测肠道上皮细胞紧密连接蛋白ZO-1的表达。结果与C组比较,T组、D组和D+A组血浆E和NE浓度、血清FITC-Dex浓度和DAO活性升高,肠组织DAO活性降低,ZO-1表达下调(P<0.05);与T组比较,D组血浆E及NE浓度、血清FITC-Dex浓度和DAO活性降低,肠组织DAO活性升高,ZO-1表达上调(P<0.05);与D组比较,D+A组血浆E及NE浓度、血清FITC-Dex浓度和DAO活性均升高,肠组织DAO活性降低,ZO-1表达下调(P<0.05)。结论α2受体参与了右美托咪定改善TBI大鼠肠道屏障功能障碍的过程。Objective To evaluate the role of α2 receptor in dexmedetomidine-induced improvement in intestinal barrier dysfunction in rats with traumatic brain injury(TBI).Methods Forty clean-grade healthy Wistar rats,aged 12 weeks,weighing 200-250 g,were divided into 4 groups(n=10 each)using a random number table method:control group(group C),TBI group(group T),dexmedetomidine group(group D)and dexmedetomidine plus atipamezole group(group D+A).Traumatic brain injury model was established by a 20 g weight free fall impact method in chloral hydrate-anesthetized rats.At 30 min before establishing the model,rats were fed 4.0 kD fluorescein-isothiocyanate-conjugated dextran(FITC-Dex)60 mg/100 g.Dexmedetomidine 50μg/kg was injected intraperitoneally at 30 min before establishing the model in D and D+A groups.Atipamezole 500μg/kg was injected intraperitoneally at 10 min before injecting dexmedetomidine in group D+A.Blood samples from common carotid artery were obtained at 6 h after establishing the model for determination of concentrations of epinephrine(E)and norepinephrine(NE)in plasma(by enzyme-linked immunosorbent assay),concentrations of FITC-Dex in serum(using fluorescence spectrophotometry)and activity of diamine oxidase(DAO)in serum(by colorimetry).The small intestine was removed for determination of DAO activity(by colorimetry)and for detection of the expression of tight junctional protein zonula occludens-1(ZO-1)in the intestinal mucosa of epithelial cells.Results Compared with group C,the concentrations of E and NE in plasma,and FITC-Dex concentration and DAO activity in serum were significantly increased,the DAO activity in intestinal tissues was decreased,and the expression of ZO-1 was down-regulated in T,D and D+A groups(P<0.05).Compared with group T,the concentrations of E and NE in plasma,and FITC-Dex concentration and DAO activity in serum were significantly decreased,the DAO activity was increased,and the expression of ZO-1 was up-regulated in group D(P<0.05).Compared with group D,the concentrations of E and N

关 键 词：受体 肾上腺素能α 右美托咪啶 颅脑损伤 肠黏膜 

分 类 号：R614[医药卫生—麻醉学]