GlgC基因种子特异过表达载体的构建及对甘蓝型油菜的转化  

Construction of Seed-specific Overexpression Vector with GlgC and Its Utilization in Rapeseed(Brassica napus L.)

作　　者：赵凯琴 罗延青[2] 俎峰[2] 王敬乔[2] 李劲峰[2] 陈苇[2] 张云云[2] 杨清辉[1] ZHAO Kai-qin;LUO Yan-qing;ZU Feng;WANG Jing-qiao;LI Jin-feng;CHEN Wei;ZHANG Yun-yun;YANG Qing-hui(College of Agronomy and Biotechnology,Yunnan Agricultural University,Yunnan Kunming 650201,China;Cash Crops Research In-stitute,Yunnan Academy of Agricultural Sciences,Yunnan Kunming 650205,China)

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201 [2]云南省农业科学院经济作物研究所,云南昆明650205

出　　处：《西南农业学报》2020年第2期217-223,共7页Southwest China Journal of Agricultural Sciences

基　　金：国家重点研发计划项目西南地区强优势油菜杂交种的创制与应用(2016YFD0101305);云岭产业技术领军人才。

摘　　要：【目的】通过表达外源GlgC基因增加甘蓝型油菜种子淀粉积累,从而可能增加含油量,为油菜高含油量育种提供一种潜在的新途径。【方法】本研究将定点突变的大肠杆菌AGPase基因GlgC分别和2个种子特异表达启动子DOF(拟南芥球形胚阶段启动子)、Cruc(油菜种子特异蛋白启动子)重组,构建2个GlgC过表达载体pMB-DOF-TG和pMB-Cruc-TG。利用农杆菌介导法转化甘蓝型油菜,获得转基因植株。【结果】PCR及半定量RT-PCR检测结果显示,GlgC基因已导入油菜再生植株基因组并获得表达。【结论】获得了甘蓝型油菜转GlgC基因阳性株,为利用遗传改良技术实现上述途径在油菜育种中的应用奠定前期基础。【Objective】Using the exogenous GlgC gene may increase rapeseed starch accumulation,which can provide a potential new way for rapeseed breeding with high oil content.【Method】The GlgC gene constructed two plant expression vector named pMB-DOF-TG and pMB-Cruc-TG,which was introduced into Agrobacterium tumefaciensLBA4404 and expressed itself into Brassica napus plants.【Result】The results indicated that the construction of the GlgC gene plant expression vector was successful and useful,which was proved by moleculer de-tection.【Conclusion】Positive transgenic GlgC lines were obtained,which laid a foundation for the application of genetic improvement tech-nology in rapeseed breeding.

关 键 词：GlgC基因 启动子 植物表达载体 转基因 甘蓝型油菜 

分 类 号：S565[农业科学—作物学]