四季蜜龙眼FLOWERING LOCUS T(FT)同源基因克隆及其表达分析  

Molecular Cloning of FLOWERING LOCUS T(FT) Gene and Its Expression in Sijimi Longan

作　　者：邱宏业 朱建华[1,3] 丁峰 潘介春 秦献泉[1,3] 徐宁[1,3] 李鸿莉 李冬波[1,3] 彭宏祥 侯延杰[1,2] 张树伟 QIU Hong-yel;ZHU Jian-hual;DING Fengl;PAN Jie-chun;QIN Xian-quan;XU Ning;LI Hongil;LI Dong-bo;PENG Hong-xiang;HOU Yan-jie;ZHANG Shu-wei(Horticultural Research Institute,Guangxi Academy of Agricultural Sciences,Guangxi Nanning 530007,China;Guangxi Key Lab of Crops Genetic Improvement and Biotechnology,Guangxi Nanning 530007,China;Nanning Investigation Station of South Subtropical Fruit Trees,Ministry of Agriculture,Guangxi Nanning 530007,China;Guangxi University,Guangxi Nanning 530004,China)

机构地区：[1]广西农业科学院园艺研究所,广西南宁530007 [2]广西作物遗传改良重点开放实验室,广西南宁530007 [3]农业部南宁南亚热带果树科学观测实验站,广西南宁530007 [4]广西大学,广西南宁530004

出　　处：《西南农业学报》2020年第2期224-232,共9页Southwest China Journal of Agricultural Sciences

基　　金：国家现代农业产业技术体系广西创新团队建设专项(nycytxgxcxtd-01);广西科技重大专项(桂科AA17204045-4,桂科AA17204097,桂科AA17204097-1);广西农业科学院科技发展基金项目(桂农科2015YT50,桂农科2015YT50)。

摘　　要：【目的】克隆四季蜜龙眼叶片开花基因FLOWERING LOCUS T(FT)表达并进行生物信息学分析,为探讨生长调节剂调控四季蜜龙眼夏季成花机理打下基础。【方法】以经多效唑(PP_(333))和乙烯利处理的四季蜜龙眼成熟叶片为试验材料,采用同源克隆技术克隆其FT基因的cDNA全长序列,预测该基因及其编码蛋白的理化性质,并通过实时荧光定量PCR分析四季蜜龙眼成花过程不同时期的FT表达情况。【结果】克隆获得2个四季蜜龙眼FT同源基因cDNA全长序列,分别命名为DlFT1和DlFT2,序列长度分别为755和629 bp,开放阅读框(ORF)均为525 bp,各编码174个氨基酸,二者所编码的蛋白存在24个氨基酸残基差异。四季蜜龙眼叶片中的DlFT1和DlFT2基因在四季蜜龙眼夏季成花过程中可能发挥关键作用,且DlFT2基因的表达量显著上升(P<0.05),DlFT2与成花关系更紧密。【结论】2个四季蜜龙眼FT同源基因DlFT1和DlFT2均属于PEBP基因家族的FT-Like蛋白亚家族,在PP_(333)和乙烯利诱导四季蜜龙眼成花转变过程中发挥关键作用,且DlFT2起着主导作用。【Objective】Cloning Sijimi longan leaves and flower related gene FLOWERING LOCUS T(FT) and conducting bioinformatics analysis lay a foundation for studying growth regulators role in regulating mechanism of summer flowering of Sijimi longan.【Method】The FT gene was cloned using homology-based cloning technology from Sijimi longan leaves which processed with PP333 and ethephon. The object of this study is to clone the full-length cDNA sequence of the FT,predict the gene and its physicochemical properties of the corresponding encoded protein,different periods in the expression of FT in Sijimi longan were compared using real-time quantitative PCR.【Result】Two FT homological genes of cDNA with full length were abstained by means of cloning in this study. They were named as DlFT1 and DlFT2,the full length of DlFT1 and DlFT2 cDNA were 755 and 629 bp. Analyzing showed that the ORF of both DlFT1 and DlFT2 were 525 bp with 174 codes. There was 24 amino acid redisues different between coding proteins of the two. Result of real time fluorescence quantitative showed that DlFT1 and DlFT2 in the leaves played a key role in the process of flowering of Sijimi longan,DlFT2 gene expression significantly increased(P<0.05),and DlFT2 played the leading role.【Conclusion】FT homology genes DlFT1 and DlFT2 belonged to PEBP subtribe of FT-Like family,and they play a key role in PP333 and ethephon inducing Sijimi longan flower transformation,and DlFT2s played the leading role.

关 键 词：四季蜜龙眼 FT基因 生物信息学分析 实时荧光定量PCR 

分 类 号：S667.2[农业科学—果树学]