不同稻田栽培模式下土壤细菌群落多样性分析  

Analysis on Diversity of Soil Bacterial Community Under Different Paddy Field Cultivation Patterns

作　　者：宋宇[1] 王鹏[1] 韦月平[1] SONG Yu;WANG Peng;WEI Yue-ping(Eastern Liaoning University,Liaoning Dandong 118003,China)

机构地区：[1]辽东学院,辽宁丹东118003

出　　处：《西南农业学报》2020年第2期263-267,共5页Southwest China Journal of Agricultural Sciences

基　　金：2018年中央财政专项创新团队建设(8888-851800501)。

摘　　要：【目的】本文探讨了不同稻田栽培模式下土壤细菌群落组成和多样性的变化。【方法】利用高通量测序技术对河蟹共作稻田(SDHXZ)、非洲雁共作稻田(SDFYZ)和单作稻田(SDKBZ)3种栽培模式表层土壤细菌进行高通量测序及分析。【结果】测序共获得240 070个有效序列,3157个OTU,48个门,115个纲,224个目,391个科,721个属和1343个种。Venn图显示3个处理组共有的细菌OTU数是1525个,SDFYZ特有OUT数最多为357个,SDHXZ的ace指数和shannon指数均高于SDFYZ和SDKBZ,说明SDHXZ土壤细菌多样性和丰富度高于其他2个处理。稻田土壤细菌多样性与pH和氨态氮相关性较大,丰富度则与硝态氮呈正相关。3个处理的优势菌门和优势菌属基本相同。PCoA分析图显示3个处理的土壤细菌群落结构差异较大。【结论】SDHXZ表层土壤细菌群落多样性和丰度最高,具有更好的土壤细菌生态基础。该研究可为合理选择水稻栽培模式提供一定理论支持。【Objective】The composition,structure and diversity of soil bacterial community under different paddy field soil cultivation pat-terns were discussed in this paper.【Method】The technology of high-throughput sequencing on Illumina Miseq platform combined with relat-ed bioinformatics analysis was adopted to investigate the field soil bacteria on rice-crab paddy field,rice-African geese paddy field and mono-culture paddy field.【Result】240070 effective sequences,3157 OTU,48,115 classes,224 orders,391 families,721 genera and 1343 species were obtained.Venn chart showed that the number of common bacteria OTUs for three processing groups were 1525.The specific OTU number for SDFYZ was 357,which was the largest.Both ace index and shannon index of SDHXZ were higher than those of SDFYZ and SDK-BZ,indicating that soil bacterial diversity and richness of SDHXZ were higher than those of the other two treatments.The diversity of soil bacteria in the paddy field was largely related to the relationship between the pH and the ammonia nitrogen,and the richness of the soil is positively related to the nitrate nitrogen.The dominant flora and dominant genus of the three treatments were basically the same.PCoA analy-sis showed that there were significant differences in soil bacterial community structure among the three treatments.【Conclusion】SDHXZ has the highest diversity and abundance of bacterial community in topsoil,and has a better microbial ecological base.This study can provide some theoretical support for the rational selection of rice cultivation pattern.

关 键 词：高通量测序 细菌群落多样性 河蟹共作稻田 非洲雁共作稻田 单作稻田 

分 类 号：S154.3[农业科学—土壤学]