种球包衣剂防治魔芋软腐病试验研究  

Treatment of Amorphophallus konjac Soft Rot Disease by Bulbs Coating Chemicals

作　　者：杨群辉[1] 何翔 杨佩文[1] 毕云青[1] 张庆[1] 曾波[3] 任思宇[3] 施竹凤 杨明英[1] 谢美华 YANG Qun-hui;HE Xiang;YANG Pei-wen;BI Yun-qing;ZHANG Qing;ZENG Bo;REN Si-yu;SHI Zhu-feng;YANG Ming-ying;XIE Mei-hua(Agricultural Environment and Resources Institute,Yunnan Academy of Agricultural Sciences,Yunnan Kunming 650205,China;College of Plant Protection,Yunnan Agricultural University,Yunnan Kunming 650201,China;Yunnan Chemical Research Institute,Yun-nan Kunming 650228,China;Department of Chemistry and Life Science,Chuxiong Normal University,Yunnan Chuxiong 675000,China)

机构地区：[1]云南省农业科学院农业环境资源研究所,云南昆明650205 [2]云南农业大学植物保护学院,云南昆明650201 [3]云南省化工研究院,云南昆明650228 [4]楚雄师范学院化学与生命科学学院,云南楚雄675000

出　　处：《西南农业学报》2020年第2期313-318,共6页Southwest China Journal of Agricultural Sciences

基　　金：云南省科学技术厅创新引导与科技型企业培育计划-科研院所技术开发研究专项(2019DC012)。

摘　　要：【目的】明确一种魔芋种球包衣剂的防病促生效果,为魔芋产业的绿色稳定发展提供数据和理论支撑。【方法】本研究设置6个不同处理,魔芋播前对其种球进行预处理,定期考察使用种球包衣剂对魔芋出苗率、生长性状、产量、软腐病发病率及植株死亡率的影响,并利用实时荧光定量PCR(Real-time quantity PCR, RT-qPCR)技术,对土壤中的魔芋软腐病菌进行定量检测。【结果】使用种球包衣剂能有效提高魔芋的出苗率、产量并促进植株生长,软腐病的发病率及植株的死亡率均表现:固型种球包衣剂+77%氢氧化铜可湿性粉剂>固型种球包衣剂>液型种球包衣剂+77%氢氧化铜可湿性粉剂>液型种球包衣剂>77%氢氧化铜可湿性粉剂>CK。RT-qPCR检测结果表明,土壤中魔芋软腐病菌的最低检测浓度为5×10^4 CFU/mL。【结论】使用种球包衣剂能有效减少魔芋软腐病菌的数量,显著降低魔芋软腐病的发病率和植株死亡率(P<0.05)。【Objective】The present paper aimed to clarify the effect of disease prevention and growth promotion on Amorphophallus konjac by using bulbs coating chemicals,provide data support for the sustainable development of Amorphophallus konjac industry.【Method】In this study,four different experimental treatments were set up,and the tubers were treated before the sowing.The effects of using bulbs coating chemicals on the seedling rate of Amorphophallus konjac,the disease incidence and mortality of its soft rot disease in the test plot were regu-larly researched.And real-time quantitative PCR(RT-qPCR)technique was developed and used to quantitatively detect Erwinia carotovora subsp.carotovora in soil.【Result】Using bulbs coating chemicals could effectively improve the seedling rate and plant growing.The result showed solid bulbs coating chemicals>liquid bulbs coating chemicals>72%agricultural streptomycin>CK on the disease incidence and mortality of Amorphophallus konjac soft rot disease.The lowest concentration of Erwinia carotovora subsp.carotovora in soil detected by the developed system was 5(10^4 CFU/mL.【Conclusion】The number of Erwinia carotovora subsp.Carotovora,the incidence of konjac soft rot and plant mortality could be significantly reduced by using bulbs coating chemicals or antibiotic pestside(P<0.05).

关 键 词：魔芋 软腐病 种球包衣剂 RT-QPCR 

分 类 号：S632[农业科学—蔬菜学]