高效液相色谱非接触电导检测法测定头孢噻肟钠的含量  

Determination of Cefotaxime Sodium by High Performance Liquid Chromatography with Contactless Conductivity Detection

作　　者：刁富城 周勰[2] 陈缵光[2] Diao Fucheng;Zhou Xie;Chen Zuanguang(Guangzhou Baiyunshan Pharmaceutical Holdings Co.,Ltd.,Baiyunshan Chemical Pharmaceutical Factory,Guangzhou 510515;School of Pharmaceutical Sciences,Sun Yat-sen University,Guangzhou 510006,China)

机构地区：[1]广州白云山医药集团股份有限公司白云山化学制药厂,广东广州510515 [2]中山大学药学院,广东广州510006

出　　处：《广东化工》2020年第5期185-186,201,共3页Guangdong Chemical Industry

摘　　要：目的:建立高效液相色谱非接触电导检测法测定头孢噻肟钠含量的新方法。方法:采用AquasilC18色谱柱;流动相:乙腈︰水(5︰95);流速:1.0 mL/min;进样量:10μL,检测器:非接触电导检测器(激发频率60 kHz,激发电压60Vp-p)。结果:头孢噻肟在0.51~3.04μg进样量范围内与峰面积呈良好线性关系(r=0.9997);RSD(n=6)为0.8%;平均回收率(n=9)为100.4%。结论:本方法简单便捷、重复性好、灵敏度高、快速准确,适用于头孢噻肟钠的含量测定。Objective:To establish a new method for the rapid determination of cefotaxime sodium by high performance liquid chromatography with contactless conductivity detection.Methods:The method was performed by using a colmn of Aquasil C18,with the mobile phase of 5%acetonitrile aqueous solution,at flow rate of 1.0 mL/min,and jnjection volume of 10μL,determination of cefotaxime sodium by contactless conductivity detection(excitation frequency:60 kHz,excitation voltage:60Vp-p).Results:The linear range of cefotaxime sodium was 0.51~3.04μg(r=0.9997)and the average recovery was 100.4%(n=9)while repeatability RSD was 0.8%(n=6).Conclusion:The method is convenient,reproducible,sensitive,rapid and accurate,and is suitable for the rapid determination of cefotaxime sodium.

关 键 词：高效液相(HPLC) 非接触电导检测(CCD) 头孢噻肟钠 含量测定 

分 类 号：R927[医药卫生—药学]