赶黄草黄酮浸膏的制备工艺研究  

Study on the Preparation Technology of Flavonoid Extract from Penthorum Chinense Pursh

作　　者：肖荣明 冯婷婷 杨善彬[1,2] 王晴晴 邓乾华 张雪华 李想 王晓楠 李霄 李标 蒲道俊 Xiao Rongming;Feng Tingting;Yang Shanbin;Wang Qingqing;Deng Qianhua;Zhang Xuehua;Li Xiang;Wang Xiaonan;Li Xiao;Li Biao;Pu Daojun(College of Chemistry,Chongqing Normal University,Chongqing 401331;Engineering Research Center for Biotechnology of Active Substances,Ministry of Education,Chongqing Normal University,Chongqing 401331;Southwest Pharmaceutical Co.,Ltd.,Chongqing 400038,China)

机构地区：[1]重庆师范大学化学学院,重庆401331 [2]重庆师范大学活性物质生物技术教育部工程研究中心,重庆401331 [3]西南药业股份有限公司,重庆400038

出　　处：《广东化工》2020年第4期27-29,共3页Guangdong Chemical Industry

基　　金：重庆市高校创新团队项目(CXTDX201601018);活性物质生物技术教育部工程研究中心开放基金项目(No.20150408,AS201903);重庆师范大学成果转化项目基金(15XZH08);重庆师范大学博士启动项目(12XLB006);重庆市大学生创新创业训练计划项目(S201910637014);重庆市社会事业与民生保障科技创新专项项目(cstc2015shmszx80060)。

摘　　要：采用冷浸-超声提取技术,以浸膏得率、槲皮素占比为指标,正交优选固液比、提取温度、提取时间对赶黄草黄酮浸膏提取效果的影响,并与其他提取方法进行比较。结果表明赶黄草黄酮浸膏的最佳制备工艺为:以纯化水为提取溶剂,固液比为1∶16,冷浸2 h,60℃下超声提取60 min;65℃下减压浓缩(-0.07 MPa),50℃下烘干。此条件下浸膏得率、槲皮素占比分别为99.42 mg/g、12.03 mg/g,均高于超临界CO2萃取法、煎煮法、回流提取法。Using cold soaking-ultrasonic extraction technology, with extraction yield of flavonoid extract and quercetin proportion as indexes, the effects of solid-liquid ratio, extraction temperature and extraction time on the extraction efficiency of flavonoid extract were optimized by orthogonal test, and compared with the other extraction methods. The experimental results showed that the optimum preparation technology of flavonoid extract was as follows: purified water as extraction solvent, solid-liquid ratio is 1∶16, cold soaking for 2 h, ultrasonic extraction at 60 ℃ for 60 min;decompression concentration(-0.07 MPa) at 65 ℃, drying at 50 ℃. Under these conditions, the extraction yield of flavonoid extract and quercetin proportion were 99.42 mg/g and 12.03 mg/g respectively, which were higher than the other methods including supercritical CO2 extraction, decoction and reflux extraction.

关 键 词：赶黄草 黄酮浸膏 槲皮素 正交试验 

分 类 号：R284.2[医药卫生—中药学]