龙血素A对瘢痕疙瘩成纤维细胞生长作用研究  

Study on the Effects of Loureirin A on Keloid Fibroblast Growth Function

作　　者：马卉[1] 张润田[1] 李航[2] 黄敏[1] 陈广山[1] 刘洋 曲天歌 包海兰 周琳[1] 赵艳[1] 段行武[1] 李玲玲[1] MA Hui;ZHANG Runtian;LI Hang;HUANG Min;CHEN Guangshan;LIU Yang;QU Tiange;BAO Hailan;ZHOU Lin;ZHAO Yan;DUAN Xingwu;LI Lingling(Department of Dermatology,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing,100700,China;Department of Dermatology,Peking University First Hospital,Beijing,100034,China;Beijing Shijitan Hospital Affiliated to Capital Medical University,Beijing 100038,China;Dongfang Hospital of Beijing University of Traditional Chinese Medicine,Beijing 100078,China;Department of Integrated Traditional Chinese and Western Medicine,People′s Hospital of Inner Mongolia Autonomous Region,Hohhot 010017,China)

机构地区：[1]北京中医药大学东直门医院皮肤科,北京100700 [2]北京大学第一医院皮肤性病科,北京100034 [3]首都医科大学附属北京世纪坛医院,北京100038 [4]北京中医药大学东方医院教育处,北京100078 [5]内蒙古自治区人民医院中西医结合科,呼和浩特010017

出　　处：《世界中医药》2020年第6期827-831,共5页World Chinese Medicine

基　　金：中央高校基本科研业务费专项资金资助项目(2019-JYB-JS-050)。

摘　　要：目的:研究不同浓度龙血素A对瘢痕疙瘩成纤维细胞增殖、凋亡、迁移能力的影响以及对Col-I、Col-III、α-SMA、Bax、Caspase-3蛋白表达水平的影响。方法:1)不同浓度龙血素A作用于瘢痕疙瘩成纤维细胞,用CellTiter-Blue活力检测试验检测龙血素A对瘢痕疙瘩成纤维细胞增殖的影响。2)流式细胞术检测龙血素A对瘢痕疙瘩成纤维细胞凋亡的影响。3)划痕实验检测龙血素A对瘢痕疙瘩成纤维细胞迁移的影响。4)Western-Blot测定龙血素A对瘢痕疙瘩成纤维细胞Col-I、Col-III、α-SMA、Bax、Caspase-3蛋白水平的影响。结果:龙血素A可抑制瘢痕疙瘩成纤维细胞增殖、迁移并诱导其凋亡,同时减少COL-I、COL-III、α-SMA蛋白表达,并促进Bax、Caspase-3蛋白表达,并且均呈现剂量依赖性。结论:龙血素A可以调控瘢痕疙瘩成纤维细胞增殖与凋亡的平衡、减少细胞外基质沉积,从而可能对KD发挥一定的治疗作用。Objective:To study the effects of different concentrations of Loureirin A on the proliferation,apoptosis and migration of keloid fibroblasts and the protein expression levels of Col-I、Col-III、α-SMA、Bax、Caspase-3.Methods:1)Different concentrations of L-Auxin A act on keloid fibroblasts.CellTiter-Blue vitality test was used to test the effects of L-Auxin A on keloid fibroblast proliferation.2)Flow cytometry was used to detect the effects of L-Auxin A on keloid fibroblasts apoptosis.3)Scratch test was used to detect the effects of borneol A on keloid fibroblast migration.4)Proteins expression levels of Col-I、Col-III、α-SMA、Bax、Caspase-3 were detected by Western-Blot.Results:Loureirin A can inhibit the proliferation and migration of keloid fibroblasts,and induce their apoptosis,reduce the protein expression of Col-I、Col-III、α-SMA and promote the protein expression of Bax and caspase-3,and there is a dosage dependent relationship.Conclusion:Loureirin A can regulate the balance of keloid fibroblast proliferation and apoptosis,reduce the deposition of extracellular matrix,so it may play a certain therapeutic effects on keloid.

关 键 词：龙血素A 瘢痕疙瘩 成纤维细胞 

分 类 号：R285.5[医药卫生—中药学]