荧光猝灭体系测定谷胱甘肽  

Determination of Glutathione by Fluorescence Quenching System

作　　者：唐瑞韩 刘丹[1] 张心悦 TANG Ruihan;LIU Dan;ZHANG Xinyue(Chemistry and Chemical Engineering School of Sichuan University of Arts and Sciences,Dazhou Sichuan 635000,China)

机构地区：[1]四川文理学院化学化工学院,四川达州635000

出　　处：《四川文理学院学报》2020年第2期14-17,共4页Sichuan University of Arts and Science Journal

基　　金：四川省教育厅自然科学基金重点项目(17ZA0336);大学生创新创业项目(国家级)(201810644021,201810644004)。

摘　　要：利用7-(二乙基氨基)香豆素-3-甲酸与MnO2纳米体系发生荧光猝灭,建立turn-off体系用于检测谷胱甘肽,建立一种测定还原还原型谷胱甘肽的新方法.同时考察了MnO2的浓度、pH值、温度、时间对体系的影响,并应用于实际样品中谷胱甘肽的测定.该方法具有操作简便、高灵敏度的特点.Fluorescence quenching of 7-(Diethylamino)coumarin-3carboxylic Acid and MnO2 nanometer was used to establish a turn-off system for determination of glutathione,so a new method was established for determination of glutathione.The influences of pH value,temperature and time on the system were also investigated.It was applied to the determination of glutathione in real samples.This method has the characteristics of simple operation and high sensitivity.

关 键 词：香豆素 谷胱甘肽 二氧化锰 荧光分光光度计 

分 类 号：O657[理学—分析化学]