UHPLC-MS/MS同时检测IBS-D模型大鼠血浆中痛泻要方6种成分的含量及其药动学  

Content determination and pharmacokinetics of six components of Tong-Xie-Yao-Fang in IBS-D model rat plasma by UHPLC-MS/MS

作　　者：吴映秀 张毅靖 陈昭 黄育生[3,4] 熊芬[4] 唐洪梅[4] WU Ying-xiu;ZHANG Yi-jing;CHEN Zhao;HUANG Yu-sheng;XIONG Fen;TANG Hong-mei(The First Clinical School of Guangzhou University of Chinese Medicine,Guangdong Guangzhou 510405,China;Guangdong Sec-ond TCM Hospital/Guangdong Province Engineering Technology Research Institute of TCM,Guangdong Guangzhou 510095,China;Lingnan Medical Research Center of Guangzhou University of Chinese Medicine,Guangdong Guangzhou 510405,China;The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangdong Guangzhou 510405,China)

机构地区：[1]广州中医药大学第一临床医学院,广东广州510405 [2]广东省第二中医院(广东省中医药工程技术研究院),广东广州510095 [3]广州中医药大学岭南医学研究中心,广东广州510405 [4]广州中医药大学第一附属医院,广东广州510405

出　　处：《中国医院药学杂志》2020年第4期369-374,共6页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金(编号:8167151319)。

摘　　要：目的:建立超高效液相色谱-质谱法(UHPLC-MS/MS)同时测定腹泻型肠易激综合征(IBS-D)模型大鼠血浆中6种成分(芍药苷、橙皮苷、新橙皮苷、升麻素苷、5-O-甲基维斯阿米醇苷、升麻素)的分析方法,并研究这6种成分的药动学特征。方法:采用Phenomenex-C18色谱柱(4.6 mm×50 mm,5μm),乙腈-0.1%甲酸水溶液为流动相梯度洗脱,流速0.7 mL·min^-1,ESI离子源,柱温35℃,SRM检测模式。结果:各成分的线性关系良好(r>0.9974);精密度RSD<13.89%;血浆中无内源性基质干扰,稳定性良好。6种成分的达峰时间(tmax)分别为(0.5±0),(0.36±0.06),(0.33±0),(0.44±0.08),(0.31±0.13),(0.67±0.26)h,半衰期(t1/2)分别为(6.63±1.60),(4.31±1.14),(5.43±0.88),(5.98±1.91),(7.80±4.39),(4.33±0.40)h,药峰浓度(Cmax)分别为(145.42±9.81),(166.11±4.83),(167.69±2.98),(46.29±3.88),(18.98±1.85),(374.71±7.54)ng·mL^-1。结论:该方法简便、灵敏、准确,分析速度快,可用于痛泻要方的药代动力学研究。OBJECTIVE To establish an ultra performance liquid chromatography-mass spectrometry(UHPLC-MS/MS)method for the simultaneous determination of six components(paeoniflorin,hesperidin,neohesperidin,cimicifugin,5-O-methylvisamidinoside,cimicifugin)in the plasma of rats with diarrhea-predominant irritable bowel syndrome(IBS-D)and to study the pharmacokinetic characteristics of these six components.METHODS A Phenomenex-C18 column(4.6 mm×50 mm,5μm)was used with a gradient elution of acetonitrile-0.1%formic acid in water as the mobile phase at a flow rate of 0.7 mL·min^-1(ESI ion source,column temperature 35℃,SRM detection mode).RESULTS The linear relationship of each component was good(r>0.9974);RSDs of precision was within 13.89%;there was no endogenous matrix interference in plasma,and the stability was good.The time to peak(tmax)of six components were(0.5±0),(0.36±0.06),(0.33±0),(0.44±0.08),(0.31±0.13),(0.67±0.26)h,respectively;the half-life(t1/2)of them was(6.63±1.60),(4.31±1.14),(5.43±0.88),(5.98±1.91),(7.80±4.39),(4.33±0.40)h,respectively;the peak concentration(Cmax)of them was(145.42±9.81),(166.11±4.83),(167.69±2.98),(46.29±3.88),(18.98±1.85),(374.71±7.54)ng·mL^-1 respectively.CONCLUSION The method is simple,sensitive,accurate and rapid,and can be used for the pharmacokinetic study of Tong-Xie-Yao-Fang.

关 键 词：痛泻要方 腹泻型肠易激综合征(IBS-D) UHPLC-MS/MS 药代动力学 

分 类 号：R927[医药卫生—药学]