浙江蜡梅叶片挥发油提取工艺优化及成分分析  

Extraction Optimization and Chemical Compositions of Volatile Oil from Chimonanthus zhejiangensis Leaves

作　　者：陈建烟 刘超兰 李永裕[3] 郜祥雄 吴芸芸 刘伟 CHEN Jian-yan;LIU Chao-lan;LI Yong-yu;GAO Xiang-xiong;WU Yun-yun;LIU Wei(College of Life Science,Ningde Normal University,Ningde Fujian 352100,P.R.China;The Engineering Technology Research Center of Characteristic Medicinal Plants of Fujian,Ningde Normal University,Ningde Fujian 352100,P.R.China;College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou Fujian 350002,P.R.China;Antibiotics Research and Re-evalution Key Laboratory of Sichuan Province,Sichuan Industrial Institute of Antibiotics,Chengdu University,Chengdu Sichuan 610052,P.R.China)

机构地区：[1]宁德师范学院生命科学学院,福建宁德352100 [2]福建省特色药用植物工程技术研究中心,宁德师范学院,福建宁德352100 [3]福建农林大学园艺学院,福建福州350002 [4]成都大学四川抗菌素工业研究所抗生素研究与再评价四川省重点实验室,四川成都610052

出　　处：《西部林业科学》2020年第2期24-29,共6页Journal of West China Forestry Science

基　　金：福建省自然科学基金(2016J05041);福建省教育厅中青年教师教育专项科研项目(JAT160526,JA15544);宁德师范学院人才引进项目(2015Y02);宁德师范学院校级协同创新项目(2017z02)。

摘　　要：以浙江蜡梅叶片为材料,采用水蒸气蒸馏法对其挥发油提取工艺进行优化,并采用气相色谱-质谱联用法分析其成分。结果表明,浙江蜡梅的最优提取条件为浸泡时间120min,蒸馏时间240min,NaCl浓度0.5%,料液比1︰5,在此条件下其挥发油得率为1.483%;各因素对其得率的影响大小顺序为蒸馏时间>氯化钠浓度=料液比>浸泡时间。成分分析结果表明,浙江蜡梅叶片挥发油主成分为桉叶油醇(19.26%)、樟脑(11.65%)、喇叭烯氧化物-(I)(9.10%)、芳樟醇(7.74%)、蛇麻烯(7.50%)等。浙江蜡梅叶片挥发油含量丰富,其成分主要为单萜及其衍生物。The extraction optimization and chemical compositions of volatile oil from Chimonanthus zhejiangensis M.C.Liu leaves(VOCZ)were carried out by hydrodistillation extraction and gas chromatography-mass spectrometry(GC-MS).The results showed that the maximum yield of VOCZ was 1.483%under the conditions of 240min distillation with the material/water ratio of 1︰5 and 0.5%NaCl after soaking 120min.The order of influence on yield of VOCZ was distillation time>material/water ratio=NaCl concentration>soaking time.The main compositions of VOCZ were Eucalyptol(19.26%),Camphor(11.65%),Ledene oxide-(I)(9.10%),Linalool(7.74%),Humulene(7.50%).The volatile oil was abundant in C.zhejiangensis M.C.Liu leaves and the main chemical compositions of VOCZ were monoterpene and oxygenated monoterpenes.

关 键 词：浙江蜡梅 挥发油 水蒸气蒸馏法 工艺优化 成分分析 

分 类 号：S685.99[农业科学—观赏园艺]