油菜素内酯预处理对低温胁迫下华山松幼苗生理特性的影响  

Effects of Brassinolide Pretreatment on Physiological Characteristics of Pinus armandii Seedlings under Low Temperature Stress

作　　者：刘良松 杨宏艳[1] 赵冬 冯峻 赵香云 罗正平 董云祥 马焕成[1] LIU Liang-song;YANG Hong-yan;ZHAO Dong;FENG Jun;ZHAO Xiang-yun;LUO Zheng-ping;DONG Yun-xiang;MA Huan-cheng(Key Laboratory of State Forestry Administration for Biodiversity Conservation,Southwest Forestry University,Kunming Yunnan 650224,P.R.China;Forest Seeding Workstation in Yunnan Province,Kunming Yunnan 650224,P.R.China;Wild Horse Forest Farm,Huize Yunnan 654200,P.R.China;Zixi Mountain Forest Farm,Chuxiong Yunnan 675000,P.R.China)

机构地区：[1]西南林业大学西南地区生物多样性保育国家林业局重点实验室,云南昆明650224 [2]云南省林木种苗工作总站,云南昆明650224 [3]云南省会泽县野马林场,云南会泽654200 [4]云南省楚雄市紫溪山林场,云南楚雄675000

出　　处：《西部林业科学》2020年第2期99-105,共7页Journal of West China Forestry Science

基　　金：西南林业大学木棉纤维人工林产业化培育省创新团队(2018CH014);国家自然科学基金(31560207,31260175);云南省“云岭教学名师”培育项目资助。

摘　　要：为揭示外源油菜素内酯(BR)对低温胁迫下华山松幼苗的抗寒机理,以华山松幼苗为材料,研究在低温(4℃)胁迫下,不同浓度:0mg/L(CK)、0.01mg/L(G1)、0.05mg/L(G2)、0.25mg/L(G3)、1.25mg/L(G4)的BR预处理对华山松幼苗生理特性的影响。结果表明:不同浓度的BR均可提高低温胁迫下华山松幼苗叶片的含水量和光合色素含量,提高抗氧化酶活性和渗透调节物质含量,降低丙二醛含量,且0.25mg/L的BR处理效果最佳。研究表明,外源BR能有效地缓解低温胁迫对华山松幼苗的影响,降低活性氧对其幼苗的伤害,减轻膜脂过氧化程度,提高华山松幼苗的抗寒能力。In order to reveal the mechanism of brassinolide(BR)on the cold resistance of Pinus armandii seedlings under low temperature stress,Pinus armandii seedlings were used as materials to study the effects of BR pretreatment with different concentrations by 0mg/L(CK),0.01mg/L(G1),0.05mg/L(G2),0.25mg/L(G3)and 1.25mg/L(G4)on the physiological characteristics of P.armandii seedlings under low temperature(4℃)stress.The results showed that different concentrations of BR could increase the water content and photosynthetic pigment content in leaves of P.armandii seedlings under low temperature stress,increase the antioxidant enzyme activity and osmotic regulator content,and reduce the malondialdehyde content,and 0.25mg/L BR treatment effect was the best.The study show that exogenous BR could effectively alleviate the effects of low temperature stress on P.armandii seedlings,reduce the injury of reactive oxygen species to its seedlings,reduce the degree of membrane lipid peroxidation,and improve the cold resistance of P.armandii seedlings.

关 键 词：华山松 幼苗 低温胁迫 油菜素内酯(BR) 生理特性 

分 类 号：S791.241[农业科学—林木遗传育种]